A high-throughput approach for measuring temporal changes in the interactome.
[No authors listed]
摘要
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
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基因功能
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基因
GNPDA1, LOC100128588, PDCD6IP, PDCD6, GNE, TOM1L1, TOM1, RANBP9, SMC4, UBA2, SAE1, FARSB, DNM1L, ABCF2, DPP3, HUWE1, COL4A3BP, ARPC5, ARPC4, ARPC3, ARPC1B, ACTR3, ACTR2, NUBP2, ARPC2, ACTR1B, ACTR1A, PDIA6, TRAP1, OPTN, NAMPT, RBM12, G3BP1, ABI2, TRIM28, WASF2, TXNDC9, PRMT3, PSME3, DDX39A, PSMD14, PLIN3, HNRNPR, GPHN, POP7, CDK9, STAM2, STUB1, UBE4B, SF3A1, CDKN2A, MAEA, RRAGB, TFG, NPM3, TUBB3, TUBB4A, TUBB4B, NDRG1, MYL9, RACK1, BASP1, YAP1, PRMT5, ARIH2, ACAA2, COG5, PFDN6, EIF3M, SEC23B, SEC23A, CAP2, CAP1, CRTAP, SYNCRIP, VAT1, CARM1, DDX17, HYOU1, IPO7, ATG7, SSSCA1, RNASEH2A, GLRX3, DCTN2, ANP32B, PRDX4, ARPC1A, RPP30, RPP38, ARFGEF2, CCT7, CCT4, CCT2, DRAP1, SMC2, AHSA1, PAIP1, PAICS, PDLIM5, HEXIM1, STAMBP, IVNS1ABP, TRIM16, MYL12A, IGF2BP3, PNMA2, CCT8, POLD3, CFL1, MGEA5, NUDC, PTGES3, CTSC, NUP50, HBS1L, AHCYL1, WDR4, NCKAP1, RPP40, SEC24A, HSPH1, RUVBL2, USP19, SUGT1, PAPOLA, MAGED2, COPS8, PRDX3, EHD1, POP1, C11orf58, SF3A3, HNRNPA0, TOMM34, STIP1, YWHAQ, FERMT2, COPS6, GCN1, COPS5, METAP2, ADRM1, NUDT21, OGFR, DNAJB4, HNRNPUL1, ATE1, FAF1, CDC37, NUDT5, WDHD1, STRAP, SEC23IP, SUPT16H, PROSC, AKAP10, AKAP2, RPL35, PDCD10, PACSIN2, DCTN3, XPOT, SNF8, DDX19B, TP53RK, AHNAK2, COPE, GPN1, FKBP9, ACOT7, CBX3, TWF2, CHUK, OSBPL11, VASN, CCDC124, KCTD12, CKB, TBCB, TRMT61A, WDR92, FAM129A, AP3S1, CLU, CLNS1A, LEO1, ALDH16A1, CNN2, COPA, COPB1, GNPDA2, PRRC1, NCOA7, MTPN, RPL10AP3, CRMP1, ASB6, AAMP, CRYZ, CS, MAPK14, CSE1L, CSNK2A1, CSNK2A2, CSNK2B, CDAN1, CSRP1, C18orf25, CSTB, CSTF2, SPC24, CTBP2, CTH, CTNNA1, CTPS1, SEPT10, PPP4R2, ADSL, PRUNE2, ADSS, DARS, AP1B1, AARS, AP2B1, DCK, DDB1, AP1G1, DDB2, DDX1, DDX3X, DDX5, DHX9, AES, DHX15, DFFA, DHPS, DIAPH1, DLD, DNM1, DYNC1H1, DYNC1I2, AGL, DYNC1LI2, DPYSL2, DPYSL3, DR1, DRG2, DUT, DVL2, TYMP, ECHS1, AHCY, EEF1A1, EEF1A2, EEF1B2, EEF1D, EEF1G, EEF2, EIF1AX, EIF2S1, EIF2S3, EIF4B, EIF4E, EIF4G1, EIF5, EIF5A, CC2D1B, UNC13D, PDE12, CTTN, CMYA5, ENO2, TUBB, EPB41L1, EPB41L2, EPRS, EPS15, ALAD, ETF1, ETFA, FANCD2, ALDH1B1, FARSA, FASN, FAU, HNRNPUL2, OARD1, BRAT1, FOXK1, FDPS, ALDH9A1, FEN1, ALDOA, FH, FHL2, COPZ1, COPG1, HSPA4L, DNAJC8, PUF60, FKBP3, FKBP4, FKBP5, DIS3, MAPRE1, RAB3GAP1, CCT5, NT5C2, LIMCH1, CNOT1, SNRNP200, ALDOC, XPO7, MON2, PDXDC1, ERP44, SWAP70, PEG10, KIF1B, ZZEF1, FLII, NCDN, FLNA, NUP205, RTF1, TTLL12, FAM175B, FLNB, FLNC, CYFIP1, GANAB, PSME4, JMJD6, ABCF1, ANKRD28, BOP1, DNMBP, CLUH, NUP160, ANKS1A, KIAA1033, UBR4, USP24, LARP1, NUDCD3, KIAA0368, FNTA, NCAPH, EXOSC2, SIRT1, NCS1, FNTB, PES1, MACF1, NUP188, TNPO3, DNPEP, CORO1C, CD2AP, KPNA6, RABGAP1, HSPBP1, EDC4, PLD3, ARFIP2, POLA2, GSPT2, SDF2L1, MTOR, FTH1, FTL, FUS, G6PD, EHBP1L1, XRCC6, GABPA, HYPK, RAB3GAP2, ATXN10, TNFAIP8, ARIH1, HECTD1, ASF1A, MOB4, ABHD14A, RPL36, THUMPD3, NELFB, GEMIN5, PTPN23, SAMHD1, GAPDH, GORASP2, TKFC, UBXN7, APPL1, HERC4, SZRD1, KIF1BP, GAPVD1, SERBP1, TES, GARS, GART, MYCBP, GBP1, GBP2, TIMM13, ELP4, GDI1, GDI2, NAP1L5, GFPT1, RANBP6, CHORDC1, AP3M1, PABPC1, SND1, RPUSD2, CACYBP, AMPD2, SNX5, GLA, ARFIP1, PDCD4, LSM1, DIMT1, GCLC, GCLM, EIF3K, HTATSF1, PRPF19, RABGEF1, STK39, BIN1, MAT2B, GLS, GLUL, GMDS, GNAI2, GNAI3, GNL1, GNS, GOT1, SFN, GPC1, FRYL, GPS1, GPX1, DCPS, GIT1, SNX24, BZW2, MCTS1, DBNL, CHMP4A, NR3C1, BABAM1, PDIA3, GSPT1, GSR, GSS, MSH6, GTF2A1, GTF2E1, GTF2E2, GTF2F1, GTF2F2, GTF2I, USP25, TMOD3, OLA1, REPIN1, STRN4, EEF2K, EPN1, GMPPB, GMPPA, DPP7, NRBP1, STRN3, PSAT1, UBQLN2, UBQLN1, ERO1A, HIST1H1C, HIST1H1E, HIST1H1B, H2AFZ, H3F3A, ANXA2, HSD17B10, HARS, HCFC1, HTT, HDAC1, HDGF, HDLBP, HEXA, HEXB, EHD4, ANXA6, HK1, HK2, ACACA, HMGB2, HMGB3, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPF, HNRNPH1, HNRNPK, HNRNPU, HPRT1, AGFG1, APEH, PRMT1, APEX1, HSF1, DNAJA1, HSPA1A, HSPA1L, HSPA4, HSPA5, HSPA6, HSPA8, HSPA9, HSP90AA1, HSP90AA4P, HSP90AB1, HSPD1, HSPE1, DNAJB1, IARS, IDE, IDH1, ACADM, ACTBL2, TUBB8, NUP43, IKBKB, IRF2BP2, ILF2, ILK, IMPDH1, IMPDH2, INPPL1, EIF3E, IREB2, EIF6, ARCN1, JUP, KARS, NHLRC2, KIF5B, KLC1, KPNA1, KPNB1, KPNA2, KPNA3, KPNA4, TNPO1, IPO5, KRT7, KRT17, KRT18, FAM21A, KTN1, LAMB1, LAMB2, LAMP1, HSP90AB2P, LAMP2, RPSA, ACAT2, LDHA, LDHB, LGALS1, LIG1, LMNA, LMNB1, LNPEP, ARL2, LPP, MARCKS, MAN2B1, MAP2, MAP4, MARS, MAT2A, MC1R, MCM2, MCM3, MCM4, MCM5, MCM6, MCM7, ME1, MEA1, METTL1, ASL, MRE11A, ASNA1, ASNS, MSH2, ASS1, PALM2-AKAP2, MSN, MTAP, MTHFD1, MTR, MTRR, MVD, MVK, MYH9, MYL6, MYO1E, PPP1R12A, NACA, HNRNPM, NAP1L1, NAP1L4, NARS, NASP, NUBP1, NCBP1, NCL, ATIC, ACLY, SEPT2, NEDD8, NFRKB, SNU13, ACO1, NME1, NMT1, PNP, NPM1, NRDC, YBX1, NUCB1, NUCB2, NUP88, NUP98, OSBP, ALDH7A1, OXCT1, P4HB, PA2G4, PAFAH1B1, PAFAH1B2, PAFAH1B3, PRDX1, PAK2, ATP5B, CYHR1, DCAF8, PAWR, COPS7A, PCBP1, TPRKB, ISOC1, VPS36, UBXN1, LAP3, RPS27L, DERA, CUTC, TRNT1, SH3GLB1, PCNA, SBDS, RPL26L1, COPS4, DYNC1LI1, DCTN4, IPO11, NUSAP1, HGH1, PBDC1, UBAP1, GMPR2, PCYT1A, UCHL5, EIF3L, PDE3A, PPME1, YTHDF2, PDE4D, ANKFY1, RTCB, TRMT112, CHMP5, LARS, ZC3HC1, VTA1, SCLY, ARMCX3, UFM1, LARP7, SRRT, CUTA, PDHA1, TRMT6, ATP6V1H, GET4, PDHB, LUC7L2, OTUD6B, C14orf166, GINS2, LSM8, VPS29, DNAJB11, C9orf78, STK26, KDM3B, PHAX, PEPD, PFAS, PFDN1, PFDN2, PFDN4, PFDN5, PFKL, PFKM, PFKP, PGD, PGGT1B, ATP6V1A, ATP6V1B2, SERPINE2, ATP6V1C1, ATP6V1E1, PKM, PLA2G4A, PLCB3, PLCG1, PLEC, PLOD1, PLOD2, PLS1, PLS3, POLE3, CHRAC1, DCUN1D1, NANS, POLA1, POLD1, POLD2, POLR2A, POLR2B, POLR2C, POLR2E, POLR2H, TERF2IP, TOLLIP, EXOSC4, PUS7, WDR44, LZTFL1, PAF1, PPAT, CTSA, PPIA, PPIB, PPID, ELP6, QRICH1, DPP8, NSUN2, SPDL1, RPP25, DNAAF5, UBE2R2, PPM1G, THG1L, PPP1CA, GID8, PPP1CB, PIH1D1, PPP1CC, PRPF39, USP47, PPP1R2, ATG16L1, OXR1, PPP1R7, ELP3, UBR7, PPP2CA, PPP2CB, PRMT6, PPP2R1A, PPP2R1B, RPRD1A, APPL2, MAP1S, PPP2R2A, SBNO1, FANCI, PANK4, UBA6, OGFOD1, ELP2, PPP2R5C, PPP2R5D, PPP6R3, PPP2R5E, PPP3CA, DDX19A, PPP4C, PPP3CB, ABCF3, TBC1D2, PPP5C, PPP6C, PRCP, CDV3, NAGK, PRIM2, KIF21A, TRMT1, THUMPD1, OSGEP, PRKACA, CDC37L1, NPLOC4, PPP4R3A, PRKACB, IWS1, RABL6, IPO9, TSR1, ASUN, PRKAR1A, VPS35, ARFGAP1, ENAH, PARVA, NUP133, CNDP2, SEPT11, PRKAR1B, INTS9, WDR12, PRKAR2A, DCAF6, CAND1, BRK1, ACTR10, PRKCSH, UNC45A, PRKDC, CTTNBP2NL, RCC2, NSFL1C, UBFD1, RNF20, MEPCE, PRPS1, PRPS2, PRPSAP1, PRPSAP2, CTPS2, PSAP, DIABLO, BCCIP, PSMA1, PSMA2, PSMA3, GRIPAP1, PSMA4, PSMA5, PSMA6, PSMA7, UGGT1, KCMF1, PSMB1, WRNIP1, PNO1, PSMB2, SH3GLB2, PSMB3, SMARCAD1, PSMB4, DUS3L, PSMB5, PSMB6, PSMB7, PSMB8, PARP6, KIF15, PSMC1, PSMC2, CIAPIN1, PSMC3, ANKMY2, PSMC4, PSMC5, PSMC6, PSMD1, PSMD2, PSMD3, PSMD4, PSMD5, CAMK1D, GOPC, PSMD7, PSMD8, PSMD9, PSMD10, PSMD11, CORO1B, PSMD12, PSMD13, PSME1, PSME2, PPP4R3B, PTBP1, BAG1, SPC25, SCYL1, BIRC6, LRRC47, MTA3, XPO5, TWF1, PTMA, KIAA1468, PTMS, SHTN1, CCAR2, PTPN11, LSM2, PTPN12, PURB, ACTA1, PXN, PEX5, PCYT2, PYGB, PYGL, RPRD1B, EPS15L1, SNX6, QARS, RAB1A, BCAT1, RABGGTA, RAD23A, RAD23B, RAD51, RAN, RANBP1, RANGAP1, RAP1GDS1, RARS, RBBP4, RBBP7, SCPEP1, RCN1, RCN2, RDX, RECQL, RELA, RFC2, RFC3, RFC4, C12orf10, NIF3L1, AASDHPPT, RNH1, ELAC2, ABCE1, RIC8A, FKBP10, ROCK1, RPA1, RPA2, RPA3, RPL3, RPL7A, RPL8, RPL10, RPL11, RPL13, RPL15, RPL17, RPL18, RPL18A, RPL19, RPL21, RPL22, RPL23A, RPL24, RPL26, RPL27, RPL30, RPL27A, RPL28, RPL29, RPL38, RPL39, RPLP0, RPLP1, RPLP2, RPS2, RPS3, RPS3A, RPS4X, RPS5, RPS6, RPS6KA1, RPS6KA3, RPS7, RPS8, RPS9, RPS10, RPS11, RPS12, RPS13, RPS14, RPS15, RPS15A, RPS16, RPS17, RPS18, RPS19, RPS20, RPS21, RPS23, RPS24, RPS25, RPS26, RPS27, RPS27A, RPS28, RPS29, RRM1, CLIP1, RSU1, S100A4, S100A13, SARS, RNF123, UBE2O, SH2D4A, SEC13, KIF13A, SMOC1, NCAPG, P3H1, SET, IRF2BPL, MMS19, BLMH, SRSF5, SRSF6, ATG3, SGTA, SH3GL1, SETSIP, SHMT1, COPS7B, NUCKS1, SHMT2, ACBD3, TNS3, CRTC3, GINS3, TBC1D15, KLC2, TUT1, FAM129B, WNK1, PYCRL, NME1-NME2, PHACTR4, SMS, SUMO3, SNCA, SNRPA, SNRPA1, SNRPB, SNRPB2, SNRPD1, BNIP2, SNRPD2, SNRPD3, SNRPE, SNRPF, SNX1, SNX2, SORD, SP100, SPTAN1, SPTBN1, SRP9, SRP14, SRP19, SRP68, SRP72, TROVE2, SSB, SSRP1, ST13, STAT1, STAT3, STAT5B, STAT6, SUPT5H, SURF2, TALDO1, BTF3, TARS, TBCD, TBCE, TCEA1, TCEB1, TCEB2, TCP1, DYNLT3, PRDX2, BUB1B, TGM2, BYSL, THOP1, C1QBP, TLE3, TLN1, ACTG1, TPD52, TPD52L2, TPM1, TPM2, TPM3, TPM4, TPP2, HSP90B1, CCT3, TRIP6, TSN, TSNAX, TSTA3, TTC1, DNAJC7, TTC4, TUBA4A, TUBB2A, TUBG1, TUFM, EIF3CL, PPP1R2P4, TXN, TXNRD1, TYMS, UBA1, UBE2H, UBE2I, UBE2V1, UBE3A, SUMO1, UFD1L, UGDH, UGP2, UROD, VARS, VASP, VBP1, VCL, VCP, EZR, VIM, WARS, EIF4H, XPNPEP1, XPO1, XRCC5, YWHAB, YWHAE, YWHAG, YWHAH, YWHAZ, SF1, CNBP, ZYX, CACNA1S, CSDE1, TUBA1A, USP7, NUP37, AHNAK, TSEN34, ASPSCR1, FTO, DCTPP1, WDR77, DEK, BAG6, EFHD2, BRCC3, DDX39B, FAM65A, CDC73, RNASEH2B, EFL1, AIMP2, HECTD3, IPO4, TBL1XR1, TBC1D17, MYH14, RABEP2, UBA5, MCMBP, NUP85, TCEAL4, CALD1, TUBA4B, VCPIP1, NAA15, ARMC9, PAAF1, RUFY1, WDR26, GRPEL1, CCDC6, PUS1, DCAF11, COASY, WDR61, CUL5, TSEN2, AARSD1, USP5, MLF2, CALR, ACTN4, ANP32A, CALU, TXNDC5, ANP32E, ARPC5L, RAB1B, SEH1L, CAPN1, USP11, USP9X, CAPN2, SMC1A, NAA10, CAPNS1, LAGE3, CAPZA1, CAPZA2, CAST, CAPZB, CARS, HIST1H2AB, HIST1H2BO, COG3, HIST1H4A, DYNLRB1, MAD1L1, EIF2A, TATDN1, CASP7, USP48, PSMG3, STK24, GINS4, DDI2, PYM1, VPS25, SARNP, AKT1S1, RASAL1, CUL4B, CUL4A, C14orf142, CUL3, CUL2, CUL1, TUBB6, HDGFRP2, OGT, TUBA1C, HIRIP3, ZNF503, ZFYVE19, LTV1, NUDCD1, RANBP3, UBL7, PPFIA1, IKBKG, IKBKAP, HAT1, YBX3, COPS3, API5, TNKS1BP1, AP3B1, DENR, YARS, KHSRP, RUVBL1, ASMTL, PSMG1, DYNLL1, EIF3A, EIF3B, EIF3C, EIF3D, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, SERPINH1, ACTN1, GBF1, CBS, PABPC4, IQGAP1, GMPS, GGH, TSC22D1, SQSTM1, FUBP1, ZPR1, NAE1, EIF2B3, EIF2B2, EIF2S2, MTMR2, AP1M1, TIMELESS, HERC1, FUBP3, KYNU, AP3D1, HIST1H2AG, H1FX, LIMD1, KLC4, NOL3, UBA3, SPAG9, ZNF622, RPL14, ERI1, AIP, MAP7, PAPSS2, PAPSS1, LRSAM1, CCT6A, USP14, USP8, DPP9, TRIM47, NMI, PDLIM1, PRPF4, RABEP1, ANKRD40, ARHGEF1, BTF3L4, HGS, NEK9, ZW10, BUB3, COG7, LRRFIP1, MTA2, NOLC1, NAF1, LDHAL6B, AIMP1, DTD1, FAM114A1, COPB2, ATP6V1F, NAPRT, COPS2, TRIP13, TRIP10, GTF3C4, EFTUD2, RPL23, TXNL1, TRMT10A, SLC9A3R1, PLAA, ZRANB2, TJP2, H2AFV, ATG5, ROCK2, NPEPPS, EEF1E1, BAG5, BAG3, BAG2, POLR1C, ATP6V1G1, VPS26A, BCAR1, BRE, AKAP12, PDIA4, CD44, GDA, NUP155, SEC24C, ARHGEF10, CTR9, EIF5B, TTI1, N4BP1, CLINT1, BZW1, UBE3C, CROCC, SART3, KNTC1, USP34, SLK, KIAA0101, EIF4A3, DHX38, IST1, SCRN1, CDK1, PSMD6, SEC24D, LRBA, TBC1D4, SEPT7, TELO2, KIAA0196, NCAPD2, SEC16A, OXSR1, WDR1, USP15, MVP, CDC34, RBX1, CDC42, PPP4R1
原始数据
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文献解读
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