[No authors listed]
Centrosome morphology and number are frequently deregulated in cancer cells. Here, to identify factors that are functionally relevant for centrosome abnormalities in cancer cells, we established a protein-interaction network around 23 centrosomal and cell-cycle regulatory proteins, selecting the interacting proteins that are deregulated in cancer for further studies. One of these components, LGALS3BP, is a centriole- and basal body-associated protein with a dual role, triggering centrosome hypertrophy when overexpressed and causing accumulation of centriolar substructures when downregulated. The cancer cell line SK-BR-3 that overexpresses LGALS3BP exhibits hypertrophic centrosomes, whereas in seminoma tissues with low expression of LGALS3BP, supernumerary centriole-like structures are present. Centrosome hypertrophy is reversed by depleting LGALS3BP in cells endogenously overexpressing this protein, supporting a direct role in centrosome aberration. We propose that LGALS3BP suppresses assembly of centriolar substructures, and when depleted, causes accumulation of centriolar complexes comprising CPAP, acetylated tubulin and centrin.
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HDAC5, PDCD6, RPS15P2, HUWE1, LRPPRC, FRY, TRAP1, TRIM28, CDK2, CDK3, ALYREF, CDK4, PSME3, CDK5, HNRNPR, DCAF7, CDKN1A, DNAJA2, CCNO, TUBA1B, TUBB3, TUBB4A, TUBB4B, MYL12B, TUBGCP3, RBM14, HAX1, TIMM44, TADA3, VAT1, FBLN5, DDX17, CCT7, CCT4, CCT2, PRPF8, IGF2BP1, IGF2BP3, SPINT2, CCT8, SEPT9, SDCCAG3, TUBGCP2, PPP1R13L, RUVBL2, PNPLA6, TCERG1, MAGED2, EBNA1BP2, YWHAQ, SF3B2, ZWINT, CDC37, PSIP1, WDR6, CEP250, RPL35, RDH13, EXOSC8, TUBGCP5, HAUS1, CKS1B, CKS2, SSX2IP, PARD3B, EXOSC6, CHCHD1, CLCN6, ANAPC16, NEDD1, TDRD9, CCDC43, RAVER1, HINT3, AMER1, ADGRG4, MYL6B, TCEAL2, RPL10L, CSNK2A1, CSTA, DCST1, RPS7P4, CTNNA2, CTNND1, CTPS1, FAM117B, SPICE1, RPL7AP27, SLC2A12, AMOT, DGKH, TOR1AIP2, SDE2, DDB1, UBXN2A, DDX5, DHX9, DHX15, DYNC1I2, DSC1, DSG1, DSP, EEF1A1, EEF1A2, PGAM5, LINC00189, CRTC2, EMD, MARK2, TUBB, EP300, EPRS, FBL, F7, FABP5, ACSL4, FAT1, ZNF804B, UNC5B, FAU, HNRNPUL2, FECH, FGF2, FGF3, FGFR1, XRN2, PUF60, MMRN1, SNW1, CCT5, TRIM32, NINL, FBXO21, EXOSC7, SNRNP200, SMG1, ZSWIM8, RRP1B, MRPS27, CUL9, FLG, GPATCH8, ANKLE2, FAM120A, PRRC2C, UBR4, HAUS5, OBSL1, LARP1, SRGAP2, DICER1, SEC61G, RPL13A, SRRM2, NUP62, CDC26, FTH1, FTL, XRCC6, HECTD1, MOB4, NDUFAF3, PTPN23, ZNF385A, RAB11FIP5, ANKRD17, GIGYF2, POLDIP2, PRPF31, GBAS, TIMM13, MRPL46, PABPC1, CPNE7, AGO2, GLB1, TUBGCP4, LSM3, RBMX, TNRC6A, PRPF19, PCLO, GOLGA3, IGHV3-48, MRPS28, MRPS18B, GRIN2D, ABR, GRPR, GTF2I, ANAPC2, STRN4, HOOK2, NME7, ANAPC4, SERTAD1, STRN3, PACSIN1, HIST1H1C, HADHA, HADHB, HBA1, HBB, HBD, HDAC1, ANXA5, STOML2, HNRNPA1, HNRNPA2B1, HNRNPC, HNRNPF, HNRNPH1, HNRNPU, HPCA, HPCAL1, APC, AIRE, DNAJA1, HSPA1A, HSPA1L, HSPA2, HSPA5, HSPA7, HSPA8, HSPA9, HSPB1, HSP90AA1, HSP90AB1, HSPD1, IARS, IDH3B, TUBB2B, ILF2, ILF3, GALNT18, AGRN, KIF5B, RPL13P12, HRNR, RPL27AP6, LAMA3, LAMC1, LAMP2, LGALS3BP, LGALS7, LOC400682, PRICKLE3, LYZ, MARCKS, MAD2L1, MCM7, MDM2, MFAP4, KMT2A, MOV10, MZT1, RPS26P11, TRIM37, PPP1R12B, HNRNPM, NAP1L1, NAP1L4, NCL, RPL10A, NEK2, NME1, NOP2, NPM1, YBX1, ATP5A1, DCAF8, HP1BP3, ATP5C1, PCBP1, PCBP2, MRPS16, MRPS7, PCM1, PCMT1, PCNA, MRPS2, RPL26L1, CDK17, UBR5, DDX41, ANAPC7, ARMCX3, PPIL1, FAM96B, MRPS23, DNAJB11, PEX1, PFDN2, PHKA1, PHKA2, PHKB, PHKG2, PIK3C2A, PKP1, PKP2, NUP54, MBD3, MRPL39, POLR2H, MRPS21, ANKIB1, ZFAND6, PLEKHA5, CPVL, HAUS6, NDE1, YTHDF1, HAUS4, TRMT10C, PPM1B, PTCD3, CEP192, FAM90A1, HAUS2, PPP2CA, PPP2CB, CEP55, PPP2R1A, PPP2R1B, PBRM1, RPRD1A, PPP2R2A, ATAD3A, FANCI, CCDC87, PPP2R5C, PPP2R5D, PPP6R3, PPP2R5E, PPP3CA, PPP3CB, PPP3R1, PPP6C, HAUS7, CCDC88A, CEP72, N4BP2, PRKAR1A, ENAH, PCID2, PRKDC, CTTNBP2NL, MAPK1, NSFL1C, MAPK6, MTFR1L, PRSS1, EIF4ENIF1, PSMA1, ZC3HAV1, PSMA5, PSMA6, UGGT1, PSMB1, PSMB5, MRPS22, PSMB7, PSMC4, DDX24, SLC4A10, MIB1, SIPA1L2, LRCH2, ACTA1, LY6G5B, RAD23A, RARS, ACTA2, NTN4, RBBP4, RBBP7, RBL2, CCND1, RCN1, RCN2, RFC2, RFC3, RFC4, ACTB, MRPS35, ZFAND3, RPL3, RPL4, RPL5, RPL6, RPL7, RPL7A, RPL8, RPL10, RPL11, RPL12, RPL13, RPL15, RPL17, RPL18, RPL18A, RPL19, RPL21, RPL22, RPL23A, RPL24, RPL26, RPL27, RPL30, RPL31, RPL37A, RPL38, RPLP0, RPLP2, RPS2, RPS3, RPS3A, RPS4X, RPS5, RPS6, RPS7, RPS8, RPS9, RPS10, RPS11, RPS12, RPS13, RPS14, RPS15, RPS15A, RPS16, RPS17, RPS18, RPS19, RPS20, RPS23, RPS24, RPS25, RPS26, RPS27, RRBP1, S100A4, S100A8, S100A13, SBF1, CEACAM1, MRPS14, CLSPN, MMP27, SRSF3, SRSF7, MRPS25, TCEB1P3, RPL32P18, RPS26P50, GIGYF1, CRTC3, KLC2, MRPS26, MRPS24, MRPS11, MRPS9, MRPS5, MRPL41, MRPL40, MRPL38, SKP1, MZT2A, SMARCC1, MRPS34, SMARCE1, SNRPD3, SNTB2, SORL1, SPTAN1, SPTBN1, SRI, SRP9, TRIM21, SSBP1, AURKA, STRN, SUPT6H, TCP1, BUB1, BUB1B, TFAM, TFRC, C1QBP, TP53, TPD52L2, CCT3, TTN, TUBA4A, TUBA3C, TUBB2A, TUBG1, LOC730668, UBB, UFD1L, UQCRB, MRPL49, VASP, VCP, YWHAE, ZKSCAN1, DAP3, MOGS, TUBA1A, DDA1, CAD, DDX54, NOC4L, ASPSCR1, PRRC2A, HAUS3, CDC73, MRPL24, CEP97, MAP7D3, ARMC5, MAP9, TCEAL4, CEP76, MYO15B, MZT2B, CCDC33, SIKE1, CEP290, ITPKC, CCDC6, CEP70, PDHX, COL18A1, TUBB1, CALU, CAMK2D, SHARPIN, LAS1L, SLIRP, SYMPK, CLTCL1, DGCR14, CAPN1, USP11, USP9X, CAPNS1, EPPK1, HIST1H4H, GRWD1, ATAD3B, EIF2A, CCDC8, TMPRSS13, CEP78, UQCC2, LZTS2, NEURL4, IRS4, TUBA1C, RAE1, CILP, LRCH3, AIFM2, FAM104A, PPFIBP1, PPFIA1, NIPSNAP1, FBF1, YBX3, TUBGCP6, PPFIA3, DOCK7, PIAS1, CASK, RUVBL1, EIF4EBP3, CDC23, MTMR1, IQGAP1, CDC16, CCNA2, CCNB1, HERC2, CCND2, CCND3, H1FX, CCNE1, PHLDB2, RPL14, CCT6A, SART1, PRPF4, PRPF3, CCNE2, ANKRD40, PDCD5, ARHGEF2, BUB3, DDX21, TPGS1, AURKB, TIMM50, GTF3C5, GTF3C4, GTF3C3, EFTUD2, RPL23, LONP1, H2AFV, EIF4E2, MAGED1, ADAMTS1, BAG2, AKAP12, NCOR2, CLINT1, PUM1, PPP6R2, CEP57, VPRBP, TCAF1, TBKBP1, EIF4A3, MATR3, CKAP5, CUL7, ARHGEF17, CEP350, CEP170, PJA2, TBC1D4, CDC20, SEC16A, HELZ, CDC27, RBX1, SCO2
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