[No authors listed]
Cellular morphology is an essential determinant of cellular function in all kingdoms of life, yet little is known about how cell shape is controlled. Here we describe a molecular program that controls the early morphology of neurons through a metazoan-specific zinc finger protein, Unkempt. Depletion of Unkempt in mouse embryos disrupts the shape of migrating neurons, while ectopic expression confers neuronal-like morphology to cells of different nonneuronal lineages. We found that Unkempt is a sequence-specific RNA-binding protein and identified its precise binding sites within coding regions of mRNAs linked to protein metabolism and trafficking. RNA binding is required for Unkempt-induced remodeling of cellular shape and is directly coupled to a reduced production of the encoded proteins. These findings link post-transcriptional regulation of gene expression with cellular shape and have general implications for the development and disease of multicellular organisms.
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Sf3b3, Pls3, Xpo1, Slc38a1, Rrn3, Hat1, Ogt, Foxp1, Atp6v1h, Pnrc1, Ppp1r15b, Pkn2, Rap1a, Acat1, Cse1l, Abcb7, Actb, Prdx3, Ap1g1, Arf1, Arf4, Arf5, Arhgap5, Atp2a2, Atp5a1, Atpif1, Phb2, Birc6, Calm1, Calm2, Calm3, Ctnna1, Ctnnb1, Cbx3, Cbx5, Cct4, Cct5, Cct8, Cdc42, Copa, Cox5a, Cox7a2, Dpysl2, Csnk2b, Ctsb, Dhx15, Ddx5, Dync1h1, Dst, Mapre1, Eef1a1, Eef2, Eif3a, Eif4a1, Eif4a2, Eif4g2, Gdi1, Gdi2, Gfpt1, Ggps1, Gls, Glud1, Rack1, Pdia3, H3f3b, Hdac2, Hint1, Hmgcr, Hn1, Hnrnpab, Hnrnpl, Hspd1, Hsp90ab1, Hsp90aa1, Hspa4, Hspa9, Hspe1, Id3, Kpnb1, Eif3e, Itgb1, Itm2b, Kpna4, Kpna6, Kras, Ldha, Nup155, Matr3, Mcl1, Msh6, Laptm4a, Nap1l4, Ncl, Nfib, Ogdh, Pdcd4, Slc25a3, Plaa, Ppp1cb, Ppp2ca, Psen1, Psmb1, Ptp4a2, Ptprf, Abcd3, Rab1a, Rad21, Rlim, Rpl30, Polr2a, Sub1, Sar1a, Tra2b, Sin3a, Slc16a1, Plk2, Snrpe, Sptbn1, Trove2, Eif4g1, Mcmbp, Tcp1, Ugp2, Unk, Tfg, Tsc22d1, Slc4a7, Tmpo, Top2a, Top2b, Tubb3, Tubb4a, Txn1, Uba1, Ube2b, Usp9x, Vcl, Fam49b, Eif4h, Safb, Pja2, Fbxo11, Wac, Fam13b, Ybx1, Ywhae, Ywhag, Ywhaz, Ipo9, Prrc2b, Cstf3, Csde1, Picalm, Ipo7, Herc1, Mta2, Pcbp1, Npat, Polr2h, Mllt6, Cops3, Med14, H2afy, Nup98, Rps3, Rab5a, Txnl4a, Yme1l1, Mkln1, Atp5o, Tomm70a, Mga, Lrrc58, Tnpo3, Gars, Ncbp1, Minos1, Copb2, Hnrnpdl, Hnrnpu, Ppp6r3, Rbfox3, Sdcbp, Txnl1, Arl6ip1, Ywhab, Ptges3, Pdcd10, Serinc1, Ranbp9, Park7, Hnrnph1, Usp14, Huwe1, Gpr85, Ndfip1, Vps35, Emc6, Sdhc, Ghitm, Cox7b, Commd6, Dpy30, Mgst3, Gtf2h5, Anp32e, Usmg5, Mfsd14b, Actr2, Myeov2, Bfar, Ube2g1, Cltc, Brd3, Luc7l3, Ppid, Slc38a2, Rer1, Yipf4, Rps27l, Atp2b1, Rpl22l1, Arpc4, Ociad1, Eif3h, Leprotl1, Rpa3, Stt3b, Rab14, Nufip2, Rtn4, Spcs1, March5, Cnot8, Tmem30a, Atp6ap2, Sfpq, Cul2, Pdia6, Cand1, Dhx36, Skiv2l2, Bclaf1, Dnm1l, Actr3, Opa1, Ckap5, Srrm2, Trappc8, Ip6k2, Arpc2, Chmp5, Mtmr2, Limch1, Yipf6, Sf3b1, Rbfox2, Arid1a, Cnih4, Cul4a, Kdm1a, RANBP9, DNM1L, HUWE1, ARPC4, ACTR3, ACTR2, ARPC2, PDIA6, ATP6AP2, TFG, RACK1, IPO7, CCT4, CCT8, PTGES3, YME1L1, PLK2, TXNL4A, SUB1, PRDX3, PNRC1, RER1, PDCD10, PARK7, PHB2, CBX3, LRRC58, CLTC, COPA, COX7A2, COX7B, CSE1L, CSNK2B, CSTF3, CTNNA1, CTNNB1, COPS9, CTSB, AP1G1, DDX5, DHX15, DHX36, COMMD6, DYNC1H1, DPYSL2, EEF1A1, EEF2, EIF2S3, EIF4A1, EIF4A2, EIF4G1, EIF4G2, RPL22L1, STT3B, TUBB, ABCB7, TRAPPC8, MAPRE1, CCT5, LIMCH1, KDM1A, ARL6IP1, MGA, SF3B3, SF3B1, CBX5, LEPROTL1, SKIV2L2, SRRM2, TNPO3, RBFOX2, KPNA6, SIN3A, GARS, GDI1, GDI2, GFPT1, GHITM, FOXP1, PDCD4, GLS, GLUD1, YIPF6, SPCS1, C16orf72, CNIH4, PDIA3, MSH6, H3F3B, HDAC2, HINT1, HMGCR, HNRNPAB, HNRNPH1, HNRNPL, HNRNPU, HSPA4, HSPA9, HSP90AA1, HSP90AB1, HSPD1, HSPE1, ID3, EIF3E, ITGB1, ARF1, ARF4, ARF5, ACAT1, KPNB1, KPNA4, KRAS, LDHA, ARHGAP5, GTF2H5, MCL1, MGST3, MKLN1, MLLT6, ATP5EP2, MINOS1, NAP1L4, NCBP1, NCL, NFIB, NPAT, ATP2A2, ATP2B1, YBX1, NUP98, OGDH, OPA1, ATP5A1, PCBP1, RPS27L, RLIM, HN1, BFAR, FAM13B, WAC, IP6K2, RTCB, CHMP5, RAB14, FAM49B, ATP6V1H, LUC7L3, SLC25A3, PLS3, ATP5O, POLR2A, GPR85, POLR2H, SLC38A2, RRN3, MARCH5, PPID, OCIAD1, PPP1CB, PPP2CA, PPP6R3, IPO9, VPS35, TMEM30A, CAND1, PKN2, PSEN1, SAR1A, PSMB1, RTN4, BIRC6, SERINC1, NUFIP2, PTPRF, ABCD3, RAB1A, RAB5A, RAD21, RAP1A, ACTB, RPA3, RPL30, RPS3, SAFB, SDCBP, SDHC, SFPQ, TRA2B, SLC16A1, SNRPE, DST, SPTBN1, TROVE2, TCP1, TMPO, TOP2A, TOP2B, TXN, UBA1, UBE2B, UBE2G1, UGP2, VCL, EIF4H, XPO1, YWHAB, YWHAE, YWHAG, YWHAZ, CSDE1, MCMBP, CALM1, BRD3, FBXO11, CALM2, PTP4A2, NDFIP1, CALM3, SLC38A1, ANP32E, USP9X, ARID1A, PICALM, EMC6, YIPF4, CUL4A, CUL2, MFSD14B, DPY30, PRRC2B, OGT, USMG5, PPP1R15B, HAT1, COPS3, UNK, EIF3A, EIF3H, TSC22D1, MTMR2, HERC1, USP14, MTA2, COPB2, MED14, CNOT8, TXNL1, PLAA, COX5A, ATPIF1, ITM2B, GGPS1, SLC4A7, H2AFY, C14orf2, NUP155, LAPTM4A, BCLAF1, MATR3, CKAP5, PJA2, TOMM70, CDC42, HNRNPDL
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