A genome annotation-driven approach to cloning the human ORFeome.
[No authors listed]
摘要
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
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基因功能
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基因
CHKB-AS1, HMGXB4, TOM1, LOC400927-CSNK1E, SF3A1, CACNG2, SLC25A17, DDX17, RFPL3S, RFPL3, RFPL2, RFPL1S, NUP50, TOB2, KDELR3, IFT27, CDC42EP1, DMC1, RABL2B, RABL2A, CHEK2, CHKB, PACSIN2, USP18, PHF21B, PIK3IP1, C1QTNF6, C22orf39, TANGO2, EMID1, ANKRD54, COMT, ADORA2A, CRKL, APOBEC3D, ZNF280B, CRYBA4, CRYBB1, CRYBB2, CRYBB3, CSF2RB, CSNK1E, CCT8L2, LINC00896, AIFM3, C22orf15, HSCB, CCDC117, HORMAD2, DUSP18, C22orf42, ENTHD1, DNAJB7, FAM109B, NFAM1, CDPF1, GTSE1-AS1, CYP2D6, ADSL, AP1B1, TMPRSS6, APOBEC3H, WBP2NL, DDT, POLR3H, CYB5R3, APOBEC3A, APOBEC3F, EWSR1, MORC2, HIC2, GRAMD4, TTLL12, MLC1, KIAA0930, PES1, PRAME, SEC14L2, RBFOX2, SEZ6L, RASD2, PATZ1, SH3BP1, TSSK2, SDF2L1, PPIL2, PITPNB, PISD, IL17RA, BRD1, BCL2L13, TFIP11, PRR14L, SERHL2, XRCC6, TMEM211, C22orf31, TBC1D22A, CBY1, SUN2, FBXO7, RHBDD3, TTLL1, SAMM50, ATXN10, TXN2, TMEM184B, SULT4A1, GGA1, RIBC2, ARFGAP3, GGT1, GGT5, TRMT2A, KCNMB3P1, CYTH4, RSPH14, CSDC2, APOBEC3C, DESI1, SGSM3, GNAZ, MCHR1, TTC28-AS1, SEC14L4, GSTT1, GSTT2, NCAPH2, YPEL1, ZDHHC8, VPREB3, H1F0, SERPIND1, HMOX1, SLC35E4, TEX33, LINC00634, IL2RB, PI4KAP2, KCNJ4, LGALS1, LGALS2, LIMK2, ARSA, MB, MCM5, MFNG, MGAT3, MIF, MN1, MPST, MYH9, NAGA, ATF4, NCF4, NDUFA6, DRG1, NF2, SNU13, ACR, OSM, ACO2, DRICH1, EIF3L, RTCB, GTSE1, MTFP1, PDGFB, MED15, UPB1, TUBA8, CECR1, PMM1, SEPT5, POLR2F, MOV10L1, DGCR8, GNB1L, PPARA, LINC01521, TUG1, FAM118A, MIOX, PEX26, TRMU, ZMAT5, SEPT3, MAPK11, EIF4ENIF1, PANX2, TOMM22, ASPHD2, MICAL3, MKL1, PVALB, RAC2, RANBP1, RANGAP1, RFPL1, APOBEC3G, RPL3, MAPK12, BID, BIK, XPNPEP3, PARVG, TBC1D22A-AS1, CERK, MRPL40, RTN4R, SLC5A1, GATSL3, SLC7A4, SMARCB1, SLC2A11, SNRPD3, SOX10, SREBF2, SSTR3, ST13, TCN2, TEF, TSPO, TIMP3, CLDN5, TST, HIRA, UBE2L3, UFD1L, UPK3A, VPREB1, XBP1, YWHAH, MPPED1, ZNF70, ZNF74, CENPM, ALG12, NOL12, CCDC134, ADM2, FOXRED2, PNPLA3, THAP7, APOL6, APOL4, APOL3, DGCR6, LZTR1, DGCR14, SYN3, CDC45, SELO, SLC25A18, L3MBTL2, TBC1D10A, HDAC10, PLA2G6, ZNRF3, TMEM191A, LDOC1L, POLDIP3, TPST2, C22orf23, PHF5A, KLHL22, DGCR6L, KIAA1656, MICALL1, THOC5, EIF3D, MTMR3, TOP3B, HPS4, P2RX6, LMF2, DERL3, RNF185, SYNGR1, RPS19BP1, SMDT1, LARGE, SNAP29, SERHL, GRAP2, PICK1, GAL3ST1, GTPBP1, APOBEC3B, RAB36, ZBED4, JOSD1, RBX1, DGCR2, SCO2
原始数据
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文献解读
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