Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
[No authors listed]
摘要
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
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基因功能
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基因
yafF, ileS, dapB, ispH, ftsA, ftsZ, folA, murE, ftsI, lspA, ftsL, acpP, murF, mraY, lpxC, murD, secA, ftsQ, secM, can, lpxB, lpxA, erpA, dapD, tsf, bamA, rseP, ispU, cdsA, dnaE, lpxD, fabZ, tilS, accA, yafN, yagG, pyrH, lptD, hemB, dxr, eno, ispF, dxs, ispA, adk, ftsK, dnaX, rplT, rpmA, entD, folD, mrdB, mrdA, nadD, fldA, glnS, lptG, cydA, pheT, metK, folC, murJ, infA, cydC, serS, lpxK, msbA, rpsA, kdsB, mukF, mukB, mukE, asnS, fabA, rplY, ribD, yceQ, rne, fabG, holB, tmk, lolE, lolD, lolC, purB, cohE, minE, minD, ribA, pth, fabD, prs, ispE, lolB, hemA, prmC, kdsA, pgsA, mqsA, ribC, topA, fabI, racR, ftsB, pyrG, frr, murC, grxD, ydfB, ydiL, lnt, thrS, pheS, infC, dicA, lptE, tsaB, murG, ftsW, aspS, argS, ribE, yefM, nrdA, gyrA, metG, yejM, nrdB, accD, fabB, zipA, ligA, hemL, gltX, rpmB, nadE, dapA, cysS, der, hisS, ispG, pgpC, tadA, rnc, era, acpS, lepB, rpoE, pssA, bamD, nadK, rplS, grpE, trmD, ffh, rpsP, trmA, csrA, mazE, suhB, lgt, ispB, coaD, thiL, pgk, fbaA, yqgF, plsC, leuS, parE, ribB, hemH, cca, holA, rpsU, tsaD, tdcF, mreC, rsmI, gapA, nusA, infB, ftsH, obgE, yhdJ, murA, lptC, lptB, mreD, accB, hemC, accC, fmt, def, tsaC, rplQ, rpsM, rpsK, rpsD, rpoA, rpsE, rpmD, rplO, secY, rplE, rpsN, rpsH, rplF, rplR, rplP, rpmC, rpsQ, rplN, rplX, rpsS, rplV, rpsC, rpsJ, rplC, rplD, rplW, rplB, rplM, rpsL, rpsG, fusA, degS, rpsB, map, trpS, yrfF, lptA, asd, mlaB, ftsE, ftsX, rpoH, ftsY, yhhQ, bcsB, glyQ, rhsJ, gpsA, waaU, spoT, gmk, gyrB, yidC, rnpA, rpmH, dnaA, dnaN, tnaB, glmS, glmU, ispD, wzyE, dapE, ubiB, ubiD, hemG, yihA, ftsN, murI, birA, murB, rplK, nusG, secE, rpoC, rpoB, rplL, rplJ, ubiA, plsB, lexA, dnaB, ssb, hemD, mreB, alsK, dut, groS, psd, orn, tsaE, rpsR, chpS, ppa, valS, folK, lptF, tyrS, dnaC, ubiJ, lolA, rpsI, prfA, folE, dfp, waaA, lpxH, rplU, proS, yhbV, secF, ribF, secD
原始数据
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文献解读
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