Global topology analysis of the Escherichia coli inner membrane proteome.
[No authors listed]
摘要
The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
基因功能
- {{$index+1}}.{{ gene }}
基因
arnE, wecF, ygaY, glvC, yfjD, cysW, ghxQ, nirC, gntU, yrdE, yaaJ, nhaA, caiT, yaaU, kefC, yabI, thiP, satP, setA, lspA, yfdC, ydfJ, bcr, mraY, wzzE, nhaB, gcd, yadH, rseP, cdsA, metI, cyoD, yciS, yagG, yahN, rclC, eutH, codB, mhpT, pqiA, dcuC, tauC, yqeG, gfcA, brnQ, phoR, proY, cvpA, yajR, cyoE, betT, cyoA, amtB, mdlB, mdlA, ygaZ, ftsK, acrB, ygaH, ybbP, fsr, ybbJ, ybbY, mscK, nuoN, fetB, ybbW, yniB, ybcI, ydgC, ydgI, macB, wbbH, fepE, cusA, yedE, pheP, fepG, cstA, fepD, yaiY, citA, mrdB, cmtA, sapC, arnC, nagE, hyfE, aer, sdhC, abrB, sdhD, lptG, hycC, cydA, arnF, cydB, zitB, pnuC, mngA, opgE, modB, ybaT, ybhI, yifK, ybhL, ybhR, ybhQ, ybhN, ybhS, yqaA, exbB, potE, ybiR, gltJ, mdfA, ybjG, gsiC, gsiD, yliE, yliF, acrD, ybjJ, ybjL, potH, ybjM, potI, murJ, ybjO, aqpZ, dmsC, cydC, focA, ycaD, ycaM, ycaP, msbA, ycaI, nuoL, elyC, ybaN, hyaA, hyaC, cbdA, ycdU, ycdT, yccF, torS, putP, pgaC, ydhU, cyoB, ycdZ, glnP, opgH, yceJ, ydjM, ptsG, lolE, lolC, yagU, ycfT, ymfA, ycfZ, potC, potB, ycgG, cvrA, dauA, ychQ, narK, narX, chaA, pgsA, rhmT, flc, narU, hofC, narI, oppC, clsA, ynfH, oppB, ychE, kch, yciC, nfrB, yciQ, tehA, sohB, pgpB, btuC, ycjF, mdtK, ycjO, ycjP, yfgO, ynaI, ydcV, ydaN, abgT, sieB, trkG, ybiO, yddG, yddA, ynbB, ynbA, ydcU, cusS, chbC, napH, ynbD, trg, ydcZ, malX, yccA, aroP, ansP, ddpC, narV, fdnI, ddpB, kdpA, yebQ, gadC, htpX, ydhC, eamA, ydeE, lsrC, copA, ynfA, rsxD, ynfM, mdtJ, tqsA, pntB, rsxA, ydgK, leuE, ydiK, yneE, sapB, ydiM, ydiN, lnt, ynjF, yciB, ydjN, ynjI, ybdG, ydjX, lsrD, entS, ydjZ, ynjC, ydjE, nupG, mmuP, puuP, yeaJ, ydjK, yccM, yeaL, yeaN, ftsW, phoQ, manY, yoaE, mntP, manZ, yobD, dsbB, yebS, yebE, yeaI, znuB, yecN, flhA, flhB, tap, tar, tyrP, ampG, fliF, ygiZ, yedA, fliP, fliR, yedI, yedQ, yedR, yedZ, yedV, yodB, shiA, yeeO, yeeR, cobS, yeeE, ydhK, plaP, yeeA, wcaD, wzzB, wzxB, yegH, wzc, dcuD, ybaL, wzxC, wcaJ, rutG, yegE, iceT, gatC, mdtC, baeS, ccmH, pntA, yegT, yafU, nupX, yehY, yohD, yohC, lysP, yeiH, psuT, fruA, setB, ampE, yejB, yejE, yejM, ccmB, lpxT, rtn, ccmC, glpT, yojI, clcA, atoE, ymfE, ypdA, kdpD, nuoJ, rbn, nuoH, nuoA, yfbS, cynX, yfbV, yfcC, hisM, yfcJ, dedA, yfcA, fecD, gtrS, emrY, sgcC, yfeO, yfeA, xapB, yfeH, cysZ, cysU, sbmA, fhuB, murP, nupC, cyoC, mntH, yfeZ, mdtH, opgC, narQ, hyfB, hyfF, yfgF, uraA, yccS, hcaT, glrK, zupT, lepB, rhtA, rseC, eamB, kgtP, afuB, yebZ, yfiN, ypjD, yfjW, marC, ygaP, ybjE, proW, alaE, ascF, emrB, qseC, clcB, ygbN, ssuC, ydeA, hisQ, ygcS, cybB, ydcO, yqcE, barA, glcA, sdaC, gudP, ygbE, envZ, ygdD, hyfD, ygdQ, arnT, lgt, lplT, yeaV, mdtI, yahC, ydhP, araE, gltK, yphA, dtpD, yqfA, etk, mscS, argO, yggT, yneF, galP, dtpA, kdpB, yqgA, pppA, pitB, yqhA, uidB, fucP, yghB, hyfC, pgaD, rsxE, ykgH, yqiJ, yqiK, pgpA, cbdB, bacA, plsY, yfjV, motA, ybfB, srlA, ttdT, ygjI, nuoK, exuT, ygjV, sstT, alx, yqjF, yqjE, yhaI, yhaH, yhaO, tdcC, garP, yqjA, agaW, agaD, yhgE, agaC, yraQ, mtr, ftsH, yhbE, csrD, yhbX, yghQ, secG, lptC, lptB, yrbG, nuoM, mlaE, nanT, aaeB, panF, mreD, yhdY, yhdX, acrF, mscL, secY, gspF, gspO, kefB, yhfK, tsgA, frlA, yhfT, arcB, yrfF, feoB, lptA, gntT, glpG, yhgN, ugpA, ugpE, livH, livM, ftsX, zntA, yhhN, yhhT, yhhS, yhhQ, nikB, nikC, yhhJ, yhiM, rbbA, dtpB, uspB, pitA, arsB, yhiD, hdeD, mdtF, yhjE, yhjD, dctA, bcsB, yhjK, gabP, bcsA, yhjV, bcsG, dppB, dppC, yhjX, eptB, yiaD, wecH, yiaA, xylH, yiaN, yiaM, yiaW, yiaV, yibI, yibH, lldP, mtlA, waaL, yiaB, yicG, gltS, yicJ, xanP, adeQ, yicL, setC, emrD, uhpC, yidK, yidI, yidH, yidG, yidE, uhpB, dgoT, uhpT, nepI, mdtL, tnaB, adeP, cbrB, bglF, pstC, pstA, atpI, atpB, atpE, kup, rbsC, hsrA, wzxE, yigM, hemY, rhtB, rhtC, ubiB, tatC, rarD, trkH, yigF, yigG, yihG, corA, yihN, yihP, yihO, yihY, fdoI, frvB, rhaT, cpxA, kdgT, fieF, yiiR, menA, glpF, emrE, yijE, frwC, eptC, yijD, secE, zraS, yjbB, psiE, xylE, malG, malF, ubiA, dgkA, dinF, ghxP, yjcC, yjcH, actP, yjcE, nrfE, nrfD, mdtO, gltP, alsC, mdtN, creC, hybB, phnE, proP, adiC, eptA, basS, yjdF, melB, dcuS, dcuB, dtpC, dsbD, cadB, yjeH, fxsA, dcuA, frdD, sugE, mscM, yjeO, yjeM, frdC, yjfL, ulaA, cycA, ytfF, ytfL, ytfT, yjfF, treB, mgtA, ccmF, yjgN, wecA, lptF, idnT, yjhB, yjjB, yjjP, gtrA, fecC, gntP, yjiG, yjiH, yjiJ, yjiN, mdtM, creD, rstB, ylaC, lgoT, tsr, opgB, tolQ, yjiY, araH, srlE, ylaB, efeU, yjiK, rcsC, hycD, ybhM, fadE, atoS, uacT, yehU, yehW, focB, mglC, yeiB, artQ, cydD, yadS, yphD, artM, xanQ, araJ, rcnA, lacY, gtrB, yjhF, dsdX, yecS, yfdV, fryC, secF, yohK, yohJ, secD
原始数据
| Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| {{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| {{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} | |||||||||||||||||||||||||||||||||||||||||||||||||
文献解读
| {{ list.authorName }} {{ list.authorName }} |
