A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.
[No authors listed]
摘要
The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
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基因功能
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基因
LSB5, RVS161, PMP1, SYP1, ELO2, PER1, ARE1, THR4, SSK22, ERS1, ABP1, CDC50, GIT1, ALR2, HAC1, HXT10, FAB1, ATG18, VPS13, ENT4, MMP1, YLL054C, AQY2, FPS1, SSK1, YEH2, RIC1, ERG3, RFU1, GAL2, EMP70, KIN2, HOG1, SRN2, NHA1, PEP3, VTA1, ENT2, THI7, LCB5, EXG1, MID2, FKS1, ARC18, ROM2, ELO3, VPS33, BLS1, CRN1, SST2, SNC1, DRS2, SYN8, GPB2, VPS8, ERP1, PRM9, SNF3, ARF1, BRE4, GCS1, ENT1, RGT2, GET3, OSH2, YDL012C, SES1, MRH1, IPT1, VPS41, DNF2, TMN2, KIN1, SAC6, ENT5, VPS64, AKR1, DPL1, SUR2, SWA2, CNL1, GGA1, SEM1, ATO3, RVS167, HKR1, SNX41, PKH3, PKH1, ITR1, SRO77, RTG3, SFT2, BOI1, ATG8, EDE1, FUI1, SLA1, CSG2, FAT1, QDR3, TAT1, ECM33, IST2, AIM3, CCZ1, CSH1, ARL1, FTH1, SDS24, RGD1, TSC10, APM3, PHO89, RTG2, COG1, SIP2, CHC1, XRN1, GPG1, SNF4, GUP1, KXD1, HNM1, PRM8, PIB2, GET1, ERG4, COG7, MTL1, GSC2, TWF1, PIL1, ASK10, SKN1, RSR1, CLC1, MVB12, CCH1, AZR1, YAP1802, YGR266W, YOR1, ASG7, CIS3, VPS35, LCB3, PBS2, LSB6, CHS6, BCK1, TOK1, PEP8, GYP6, BBC1, SYS1, GEF1, TOR1, JSN1, ENT3, DAL5, TOR2, PEX1, JEN1, SAC1, TPK3, MRP8, TGL1, MYO3, SMY1, BLI1, SFK1, ELM1, LAC1, CAP1, YPT52, BCH2, SPO14, YSR3, ENB1, PEX11, SMF1, SKM1, WSC3, ITR2, PKH2, ADH1, RTG1, INP54, PEX15, TAT2, YOL019W, TLG2, PLB3, SLG1, ROD1, AKR2, PEP12, WHI2, STD1, RSB1, VPS5, VPS21, ARF3, RAS1, INP53, VPS17, LCB4, MKK1, DGA1, HUA2, SNC2, SCP1, GPB1, BNR1, TPM2, SLM1, PRK1, SYG1, CAP2, INP51, FLO11, VPS9, TCB3, COG8, CMP2, SUR7, GSF2, ERG6, YPT7, HXT2, ERG5, EIS1, STV1, FET3, SEG1, AIP1, YPK2, MYO5, HFD1, EAR1, GYL1, ERG2, PEP5, HOR7, SCS7, BUL1, GOT1, PEX6, LEM3, CLA4, MON2, MSB3, MID1, WSC2, BOR1, ALP1, YNL208W, YNL194C, CHS1, MDG1, YCK2, INP52, RAS2, YPT53, TCB2, SNN1, END3, TPM1, MKS1, COG5, SLM2, COG6, SIW14, CRZ1, ARK1, VPS27, LRO1, ARE2, YNR048W, BIO5, AIF1, COS10, GYP5, APL5, TRE1, TGS1, YPL150W, MKK2, BEM3, ATG21, YDC1, BRO1, YTA6, PDR12, SUR1, ARL3, VPS16, PMA2, SVL3, RAD1, NCR1, LSP1, SRO7, BSP1, VPS4, ARN2, YHL044W, ARN1, WSC4, DUR3, LAG1, HSE1, VPS29, SLT2, IRE1, YPT35, GGA2, BZZ1, YCK1, YAP1801, SCH9, SKN7, SET5, SIT1, VMA3, VAC8, GIM4, MNN1, ISC1, GPA2, YPT31, TMN3, BOI2, SHO1, SCS2, YCK3, DDI1, FTR1, DNF1
原始数据
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文献解读
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