Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin.
[No authors listed]
摘要
The role of clathrin light chain (CLC) in clathrin-mediated endocytosis is not completely understood. Previous studies showed that the CLC N-terminus (CLC-NT) binds the Hip1/Hip1R/Sla2 family of membrane/actin-binding factors and that overexpression of the CLC-NT in yeast suppresses endocytic defects of clathrin heavy-chain mutants. To elucidate the mechanistic basis for this suppression, we performed synthetic genetic array analysis with a clathrin CLC-NT deletion mutation (clc1-Î19-76). clc1-Î19-76 suppressed the internalization defects of null mutations in three late endocytic factors: amphiphysins (rvs161 and rvs167) and verprolin (vrp1). In actin sedimentation assays, CLC binding to Sla2 inhibited Sla2 interaction with F-actin. Furthermore, clc1-Î19-76 suppression of the rvs and vrp phenotypes required the Sla2 actin-binding talin-Hip1/R/Sla2 actin-tethering C-terminal homology domain, suggesting that clc1-Î19-76 promotes internalization by prolonging actin engagement by Sla2. We propose that CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization.
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基因功能
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基因
VAC17, MRC1, AGP1, RER1, SAT4, RVS161, YCR016W, BPH1, SNT1, PER1, SOL2, PAT1, YFL054C, LAM5, HAC1, BUD27, DEG1, UBP6, SAP155, RMD8, ENT4, JLP1, YBT1, SDH2, CMS1, SSK1, IZH3, UBR2, RIC1, RFU1, SIC1, HOG1, AVL9, SRN2, YPS1, APS1, VTA1, PEX13, YKE2, CLB4, EST1, YPT6, RPS28B, ECI1, NKP2, MMS22, CHS5, VRP1, YLR345W, RSC2, VPS38, VID22, IKI3, VAC14, RPS29A, BER1, URA4, ECM30, HMG2, ATS1, MDM10, CNE1, PEX22, VPS8, SWD1, KIN3, YAR029W, ARF1, AAD4, PAR32, YDL157C, TMA17, GET3, NDE2, RPS16B, RPN4, NHP10, SOK1, PAA1, IPT1, SNF11, TPS2, VPS41, UBC13, TRS85, INO2, SWF1, ARO1, ENT5, HMO1, PLP1, YDR186C, HTA1, MNN10, RMD5, HEL2, MTH1, PIB1, IPK1, LSM6, RVS167, SPT3, ERD1, STP1, VPS60, ITR1, ECM21, MAP2, AVT5, MOH1, EDE1, HIR1, HHF1, FMP23, YBR056W, UBP14, BAP2, ECM8, ECM33, UBC4, SIF2, TPS1, ARL1, PEX32, AIM4, MSI1, SDS24, MCX1, ROT2, SSH1, MNT2, RTG2, SKI8, VAM7, NCS6, HOS2, RAD54, YIP5, ARO2, ITC1, SCS3, SNF4, GUP1, SGF73, PYC1, MIG1, CWH41, PIB2, KAP122, CLC1, SET2, VPS35, INO1, RPS21B, PBS2, MTC1, GZF3, IML2, PEP8, SYS1, CPR7, CBF1, OPI3, ENT3, HOM6, HIR3, SAC1, LST4, MYO3, RAD27, KTI12, YET1, ELM1, RGT1, RPL14A, YPT52, VPS51, DBP7, BCH2, SAP190, SET3, DID2, GAP1, TRK2, RHO4, SRP40, RPL40B, INO4, HMI1, MSH2, DUF1, ADH1, IRA2, SDH5, RTG1, SPE2, RRT8, PEX15, NGL1, YOL036W, ESC8, MDM12, COQ10, SIN3, PHO80, ALG6, TSR3, PET127, ROD1, SHE4, HIR2, TOM6, VHS3, VAM10, VPS5, VPS21, VAM3, INP53, CEX1, LEO1, IDH2, GET4, LCB4, LAS17, SER1, IES4, PEX27, KIN4, ABP140, NAT5, VPH1, ISW2, SNU66, SPS4, FAA1, SNC2, PUT4, SNX3, VTS1, PDE2, AMF1, FLX1, SEC28, FIS1, AGE2, TIR3, URM1, PAN1, MGA2, PML39, URA5, MDM1, NUP188, CAC2, VPS9, HMG1, DAK1, GAL80, AMD1, YML020W, ERG6, YAP1, GIS4, YML002W, YMR010W, ERG5, UBC7, PEX12, IMP2, CSM3, SAM37, SEG1, MUB1, MYO5, PSO2, MME1, SCJ1, SKY1, SAP30, CUE1, BUL1, AEP2, UBP15, GAS1, DIA1, YMR317W, SLA2, NCS2, OCA1, NST1, EOS1, LAT1, YDJ1, COX5A, IDH1, SIW14, CRZ1, HDA1, CSE2, SMM1, SSK2, COQ2, BRE5, YNR061C, YNR065C, DIP5, YPL260W, APM1, YPL225W, TCO89, NIP100, REV3, YPL150W, FRK1, UME1, HHO1, VPS30, ELP4, EEB1, RPS6A, ELP3, BTS1, LGE1, CAM1, SGF11, PMA2, TRM44, RLF2, APL4, SRO7, THP3, BRR1, ARO7, YPR063C, SPE3, TKL1, MRL1, YPR084W, YPR089W, YPR096C, CLB5, YLH47, NCA2, TPO3, HDA3, NEM1, TDA3, VPS29, RPS27B, YHI9, PIH1, SRB2, GGA2, UBA4, AIM46, RPS24A, GET2, DOT6, RAD51, SCS2, SAK1, RTR1, BEM2, YER187W
原始数据
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文献解读
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