[No authors listed]
Coordination of the multiple steps of mRNA biogenesis helps to ensure proper regulation of gene expression. The Saccharomyces cerevisiae DEAD-box protein Rat8p/Dbp5p is an essential mRNA export factor that functions at the nuclear pore complex (NPC) where it is thought to remodel mRNA/protein complexes during mRNA export. Rat8p also functions in translation termination and has been implicated in functioning during early transcription. We conducted a synthetic genetic array analysis (SGA) using a strain harboring the temperature-sensitive rat8-2 allele. Although RAT8 had been shown to interact genetically with >15 other genes, we identified >40 additional genes whose disruption in a rat8-2 background causes synthetic lethality or dramatically reduced growth. Included were five that encode components of P-bodies, sites of cytoplasmic mRNA turnover and storage. Wild-type Rat8p localizes to NPCs and diffusely throughout the cell but rat8-2p localized to cytoplasmic granules at nonpermissive temperature that are distinct from P-bodies. In some genetic backgrounds, these granules also contain poly(A)-binding protein, Pab1p, and additional mRNA export factors. Although these foci are distinct from P-bodies, the two merge under heat-stress conditions. We suggest that these granules reflect defective mRNP remodeling during mRNA export and during cytoplasmic mRNA metabolism.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
PAT1, DEG1, LOC1, RTT109, UBR2, ARP6, SWI6, BUR2, NUP2, ROM2, CCR4, SWC3, NUP60, RPL35A, NUP84, CBS1, SIT4, MTQ2, SAC3, NBP2, NUP42, REF2, HTA1, SSD1, IPK1, SWR1, LSM6, VPS72, CSG2, UBP14, SIF2, GRS1, RTC2, SWC5, YBR259W, XRN1, RAD6, DST1, SPT4, SLX9, ELP2, EFG1, RPL39, SET2, HAL5, RPA34, LSM1, ASF1, ZAP1, CTK2, TOR1, MOG1, CTK1, TMA19, MRT4, MEH1, GCN3, NUP133, BAS1, HMI1, THP1, HTZ1, RRP6, EXO1, DBP5, LEO1, FYV12, IES4, RPL34B, APQ12, URM1, NUP188, MFT1, MAC1, CIK1, GFD1, PEX6, RPS19B, LSM7, YAF9, YDJ1, HDA1, CSE2, SSE1, DIG1, PHO85, THP3, MED1, KAR3, SKI3, SBP1, SNF6, SRB2, LRP1, SPI1, UBP3, BEM2
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |