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Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity.

Nat. Struct. Mol. Biol.2012 Dec;19(12):1257-65. Epub 2012 Oct 28
Cecilia Ballaré 1 , Martin Lange , Audrone Lapinaite , Gloria Mas Martin , Lluis Morey , Gloria Pascual , Robert Liefke , Bernd Simon , Yang Shi , Or Gozani , Teresa Carlomagno , Salvador Aznar Benitah , Luciano Di Croce
Cecilia Ballaré 1 , Martin Lange , Audrone Lapinaite , Gloria Mas Martin , Lluis Morey , Gloria Pascual , Robert Liefke , Bernd Simon , Yang Shi , Or Gozani , Teresa Carlomagno , Salvador Aznar Benitah , Luciano Di Croce
+ et al

[No authors listed]

Author information
  • 1 Department of Gene Regulation and Stem Cells, Centre for Genomic Regulation (CRG), Barcelona, Spain.

摘要


Polycomb-group proteins are transcriptional repressors with essential roles in embryonic development. Polycomb repressive complex 2 (PRC2) contains the methyltransferase activity for Lys27. However, the role of other histone modifications in regulating PRC2 activity is just beginning to be understood. Here we show that direct recognition of methylated histone H3 Lys36 (H3K36me), a mark associated with activation, by the PRC2 subunit Phf19 is required for the full enzymatic activity of the PRC2 complex. Using NMR spectroscopy, we provide structural evidence for this interaction. Furthermore, we show that Phf19 binds to a subset of PRC2 targets in mouse embryonic stem cells and that this is required for their repression and for H3K27me3 deposition. These findings show that the interaction of Phf19 with H3K36me2 and H3K36me3 is essential for PRC2 complex activity and for proper regulation of gene repression in embryonic stem cells.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
antibodycell linecell typegenotypesource name
IgG_ChIPSeq(Hi)
Mus musculus GSM1019777: IgG_ChIPSeq(Hi); Mus musculus; ChIP-Seq ChIP-Seq Illumina HiSeq 2000 IgG; Abcam, #46540 E14Tg2A embryonic stem cells wild type IgG_ChIPSeq
Phf19-Repl2_ChIPSeq(Hi)
Mus musculus GSM1019776: Phf19-Repl2_ChIPSeq(Hi); Mus musculus; ChIP-Seq ChIP-Seq Illumina HiSeq 2000 Phf19 - OpenBiosystems, costum made E14Tg2A embryonic stem cells wild type Phf19_ChIPSeq
Phf19-Repl1_ChIPSeq(Hi)
Mus musculus GSM1019775: Phf19-Repl1_ChIPSeq(Hi); Mus musculus; ChIP-Seq ChIP-Seq Illumina HiSeq 2000 Phf19 - OpenBiosystems, costum made E14Tg2A embryonic stem cells wild type Phf19_ChIPSeq
IgG_ChIPSeq
Mus musculus GSM1019774: IgG_ChIPSeq; Mus musculus; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx IgG; Abcam, #46540 E14Tg2A embryonic stem cells wild type IgG_ChIPSeq
H3K27me3-ShPhf19_ChIPSeq
Mus musculus GSM1019773: H3K27me3-ShPhf19_ChIPSeq; Mus musculus; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx H3K27me3 - Millipore, #07450 E14Tg2A embryonic stem cells Phf19-depleted H3K27me3-ShPhf19_ChIPSeq