[No authors listed]
During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein distribution in mammalian cells, yeast, and C. elegans, little is known about the role of this family in vertebrate embryogenesis. We report the presence of a single point mutation in Tmed2/p24beta(1) in a mutant mouse line, 99J, identified in an ENU mutagenesis screen for recessive developmental abnormalities. This mutation does not affect Tmed2/p24beta(1) mRNA levels but results in loss of TMED2/p24beta(1) protein. Prior to death at mid-gestation, 99J homozygous mutant embryos exhibit developmental delay, abnormal rostral-caudal elongation, randomized heart looping, and absence of the labyrinth layer of the placenta. We find that Tmed2/p24beta(1) is normally expressed in tissues showing morphological defects in 99J mutant embryos and that these affected tissues lack the TMED2/p24beta(1) oligomerization partners, TMED7/p24gamma(3) and TMED10/p24delta(1). Our data reveal a requirement for TMED2/p24beta(1) protein in the morphogenesis of the mouse embryo and placenta. Copyright 2010 Elsevier Inc. All rights reserved.
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Cdk2ap1, Gcm1, Hspa5, Itga4, Nodal, Prl3d1, Shh, Ncor2, Rsrc2, Kntc1, Eif2b1, Gtf2h3, Ccdc92, Atp6v0a2, Tpbpa, Hsp90b1, Vcam1, Xbp1, B3gnt4, Hcar1, Sbno1, Mphosph9, Zfp664, Vps37b, Dnah10, Abcb9, Tmed2, Clip1, Arl6ip4, Ogfod2, Tmed7, Tmed9, Ddx55, Setd8, Tctn2, Denr, Tmed10, Zcchc8, 2810006K23Rik, Fam101a, Rilpl1, Snrnp35, Il31, Rilpl2
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