例如:"lncRNA", "apoptosis", "WRKY"

2-Bromopalmitate impairs neural stem/progenitor cell proliferation, promotes cell apoptosis and induces malformation in zebrafish embryonic brain.

Neurotoxicol Teratol. 2015 Jul-Aug ;50:53-63. Epub 2015 Jun 06
Chen Wang 1 , Xueran Chen 2 , Wei Shi 3 , Fen Wang 3 , Zhaoxia Du 3 , Xian Li 3 , Yao Yao 3 , Tong Liu 1 , Tong Shao 3 , Gang Li 4 , Aijun Hao 5
Chen Wang 1 , Xueran Chen 2 , Wei Shi 3 , Fen Wang 3 , Zhaoxia Du 3 , Xian Li 3 , Yao Yao 3 , Tong Liu 1 , Tong Shao 3 , Gang Li 4 , Aijun Hao 5
+ et al

[No authors listed]

Author information
  • 1 Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, Jinan, Shandong 250012, PR China; Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, PR China.
  • 2 Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China.
  • 3 Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, Jinan, Shandong 250012, PR China.
  • 4 Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, PR China.
  • 5 Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, Jinan, Shandong 250012, PR China. Electronic address: aijunhao@sdu.edu.cn.

摘要


2-Bromopalmitate (2BP) is a widely used palmitoylation inhibitor. Besides, it has been reported that 2BP can inhibit T-cell activation, making it a potential immunosuppressor for the treatment of autoimmune diseases. Although the important roles of palmitoylation in a neural system have been noted during the past decades, the effect of 2BP on neural development is still not very clear. In this study, we demonstrated that 25 μM-100 μM 2BP exposure caused apparent neural malformation in the presumptive brains of zebrafish embryos at 14 hpf. Further studies implied that the mRNA quantities and distributions of neural stem/progenitor cell (NSPC) markers (neurog1, sox2, and sox3) in the affected regions of 50 μM 2BP treated embryos significantly decreased. In addition, we found that 2BP impaired the NSPC proliferation at 10 hpf and 14 hpf as well as promoted cell apoptosis at 14 hpf, consistent with which the interference with FGF/ERK signaling pathway was also detected. For the first time, this study provided information about the toxicity and teratogenicity of 2BP for neural development in vivo.

KEYWORDS: 2-Bromopalmitate, FGF signaling pathway, Neural development, Neural stem/progenitor cells, Palmitoylation, Zebrafish