Functional genomic analysis of RNA interference in C. elegans.

RNA interference of target genes is triggered by double-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for we performed a genome-wide screen using an engineered sensor strain of Caenorhabditis elegans. The duanyu1615 screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/processing factors, chromatin-associated factors, DNA recombination proteins, nuclear import/export factors, and 11 known components of the duanyu1615 machinery. We demonstrate that some of these genes are also required for germline and somatic transgene silencing. Moreover, the physical interactions among these potential duanyu1615 factors suggest links to other RNA-dependent gene regulatory pathways.

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