例如:"lncRNA", "apoptosis", "WRKY"

E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions.

EMBO J.2005 Mar 23;24(6):1146-56. Epub 2005 Mar 03
Judith A Tunggal 1 , Iris Helfrich , Annika Schmitz , Heinz Schwarz , Dorothee Günzel , Michael Fromm , Rolf Kemler , Thomas Krieg , Carien M Niessen
Judith A Tunggal 1 , Iris Helfrich , Annika Schmitz , Heinz Schwarz , Dorothee Günzel , Michael Fromm , Rolf Kemler , Thomas Krieg , Carien M Niessen
+ et al

[No authors listed]

Author information
  • 1 Center for Molecular Medicine, University of Cologne (CMMC), Cologne, Germany.

摘要


Cadherin adhesion molecules are key determinants of morphogenesis and tissue architecture. Nevertheless, the molecular mechanisms responsible for the morphogenetic contributions of cadherins remain poorly understood in vivo. Besides supporting cell-cell adhesion, cadherins can affect a wide range of cellular functions that include activation of cell signalling pathways, regulation of the cytoskeleton and control of cell polarity. To determine the role of E-cadherin in stratified epithelium of the epidermis, we have conditionally inactivated its gene in mice. Here we show that loss of E-cadherin in the epidermis in vivo results in perinatal death of mice due to the inability to retain a functional epidermal water barrier. Absence of E-cadherin leads to improper localization of key tight junctional proteins, resulting in permeable tight junctions and thus altered epidermal resistance. In addition, both Rac and activated atypical crucial for tight junction formation, are mislocalized. Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation.