[Effect of the liver X receptor agonist on LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB].

AIM:To investigate the effect of the activated LXR on the expression of IRAK-4 and NF-kappaB in the inflammatory response which was induced by LPS in Kupffer cells. METHODS:The Kuppfer cells were isolated from male Kunming mice by collagen perfusion in situ. These cells were divided into four groups: normal control group, LPS treatment group, LXR agonist T0901317 treatment group, LPS and T0901317 combined treatment group. The expression of LXR, IRAK-4 and NF-kappaB gene and their protein levels were detected by real time-PCR and Western blot, and the activity of NF-kappaB was observed by EMSA. RESULTS:The levels of LXR mRNA and protein reached the highest in T0901317 group but the lowest in LPS group. The levels of IRAK4 and NF-kappaB mRNA and protein in the combined-treatment group were evidently lower than those in LPS group. The activity of NF-kappaB reached the highest in LPS group but it was lower in the combined-treatment group and T0901317 group. CONCLUSION:LXR agonists can effectively up-regulate the level of LXR gene and protein and down-regulate the levels of IRAK4 and NF-kappaB mRNA and protein expression. They can also inhibit activity of NF-kappaB.

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