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Bone marrow allograft rejection mediated by a novel murine NK receptor, NKG2I.

J. Exp. Med.2004 Jan 5;199(1):137-44
Junzo Koike 1 , Hiroshi Wakao , Yuko Ishizuka , Taka-aki Sato , Masaru Hamaoki , Ken-ichiro Seino , Haruhiko Koseki , Toshinori Nakayama , Masaru Taniguchi
Junzo Koike 1 , Hiroshi Wakao , Yuko Ishizuka , Taka-aki Sato , Masaru Hamaoki , Ken-ichiro Seino , Haruhiko Koseki , Toshinori Nakayama , Masaru Taniguchi
+ et al

[No authors listed]

Author information
  • 1 Dept. of Molecular Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuoku, Chiba City, Chiba 260-8670, Japan.
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摘要


Natural killer (NK) cells mediate bone marrow allograft rejection. However, the molecular mechanisms underlying such a rejection remain elusive. In previous analyses, it has been shown that NK cells recognize allogeneic target cells through Ly-49s and CD94/NKG2 heterodimers. Here, we describe identification and characterization of a novel murine NK receptor, NKG2I, belonging to the NKG2 family. NKG2I, which was composed of 226 amino acids, showed approximately 40% homology to the murine NKG2D and CD94 in the C-type lectin domain. Flow cytometric analysis with anti-NKG2I monoclonal antibody (mAb) revealed that expression of NKG2I was largely confined to NK and NKT cells, but was not seen in T cells. Furthermore, anti-NKG2I mAb inhibited NK cell-mediated cytotoxicity, whereas cross-linking of NKG2I enhanced interleukin 2- and interleukin 12-dependent interferon-gamma production. Similarly, the injection of anti-NKG2I mAb before the allogeneic bone marrow transfer in vivo impinged on the function of NKG2I, resulting in the enhanced colony formation in the spleen. NKG2I is a novel activating receptor mediating recognition and rejection of allogeneic target cells.