例如:"lncRNA", "apoptosis", "WRKY"

RNA pol II transcript abundance controls condensin accumulation at mitotically up-regulated and heat-shock-inducible genes in fission yeast.

Genes Cells. 2015 Jun;20(6):481-99. doi:10.1111/gtc.12239. Epub 2015 Apr 06
Norihiko Nakazawa 1 , Kenichi Sajiki 1 , Xingya Xu 1 , Alejandro Villar-Briones 1 , Orie Arakawa 1 , Mitsuhiro Yanagida 1
Norihiko Nakazawa 1 , Kenichi Sajiki 1 , Xingya Xu 1 , Alejandro Villar-Briones 1 , Orie Arakawa 1 , Mitsuhiro Yanagida 1
+ et al

[No authors listed]

Author information
  • 1 G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa, 904-0495, Japan.

摘要


Condensin plays fundamental roles in chromosome dynamics. In this study, we determined the binding sites of condensin on fission yeast (Schizosaccharomyces pombe) chromosomes at the level of nucleotide sequences using chromatin immunoprecipitation (ChIP) and ChIP sequencing (ChIP-seq). We found that condensin binds to RNA polymerase I-, II- and III-transcribed genes during both mitosis and interphase, and we focused on pol II constitutive and inducible genes. Accumulation sites for condensin are distinct from those of cohesin and DNA topoisomerase II. Using cell cycle stage and heat-shock-inducible genes, we show that pol II-mediated transcripts cause condensin accumulation. First, condensin's enrichment on mitotically activated genes was abolished by deleting the sep1(+) gene that encodes an M-phase-specific forkhead transcription factor. Second, by raising the temperature, condensin accumulation was rapidly induced at heat-shock protein genes in interphase and even during mid-mitosis. In interphase, condensin accumulates preferentially during the postreplicative phase. Pol II-mediated transcription was neither repressed nor activated by condensin, as levels of transcripts per se did not change when mutant condensin failed to associate with chromosomal DNA. However, massive chromosome missegregation occurred, suggesting that abundant pol II transcription may require active condensin before proper chromosome segregation.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
cell typechip antibodychip antibody vendormolecule subtypepassagessource namestrain
HS_Top2_ChIPSeq WCE
Schizosaccharomyces pombe GSM1611708: HS_Top2_ChIPSeq WCE; Schizosaccharomyces pombe; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx Top2-FLAG strain after temperature-shift from 20C to 36C WCE (Whole Cell Extract) Input DNA Treated by 36°C for 9min Cells cultivated in rich media Top2-3FLAG
HS_Top2_ChIPSeq IP
Schizosaccharomyces pombe GSM1611707: HS_Top2_ChIPSeq IP; Schizosaccharomyces pombe; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx Top2-FLAG strain after temperature-shift from 20C to 36C anti-Flag M2 antibody Sigma-Aldrich Immunoprecipitated genomic DNA Treated by 36°C for 9min Cells cultivated in rich media Top2-3FLAG
HS_Rad21_ChIPSeq WCE
Schizosaccharomyces pombe GSM1611706: HS_Rad21_ChIPSeq WCE; Schizosaccharomyces pombe; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx Rad21-FLAG strain after temperature-shift from 20C to 36C WCE (Whole Cell Extract) Input DNA Treated by 36°C for 9min Cells cultivated in rich media Rad21-3FLAG
HS_Rad21_ChIPSeq IP
Schizosaccharomyces pombe GSM1611705: HS_Rad21_ChIPSeq IP; Schizosaccharomyces pombe; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx Rad21-FLAG strain after temperature-shift from 20C to 36C anti-Flag M2 antibody Sigma-Aldrich Immunoprecipitated genomic DNA Treated by 36°C for 9min Cells cultivated in rich media Rad21-3FLAG
HS_Cut14_ChIPSeq WCE
Schizosaccharomyces pombe GSM1611704: HS_Cut14_ChIPSeq WCE; Schizosaccharomyces pombe; ChIP-Seq ChIP-Seq Illumina Genome Analyzer IIx Cut14-FLAG strain after temperature-shift from 20C to 36C WCE (Whole Cell Extract) Input DNA Treated by 36°C for 9min Cells cultivated in rich media Cut14-3FLAG