Evaluation of ZAR1 and SFRP4 methylation status as potentials biomarkers for diagnosis in cervical cancer: exploratory study phase I.
[No authors listed]
摘要
CONTEXT:Aberrant hypermethylation of promoter region of tumor suppressor genes could be used as cancer biomarkers. OBJECTIVE:To test methylation status of ZAR1 and SFRP4 promoter regions as potentials biomarkers for diagnosis of preneoplastic and neoplastic lesions of cervix. MATERIALS AND METHODS:Cytobrush samples were evaluated by Methylation specific PCR (MSP) and quantitative MSP (qMSP). RESULTS:ZAR1 and SFRP4 methylation frequency increased as the grade of lesion increased and the differences between normal and cervical cancer (CC) are statistically significant (pâ<â0.0001). qMSP showed higher ZAR1 and SFRP4 methylation levels in cancer than normal epithelia (pâ<â0.001) and preneoplastics lesions (pâ<â0.01). DISCUSSION:qMSP quantify methylation levels and have high sensitivity and specificity. CONCLUSION:ZAR1 and SFRP4 qMSP could be used as potential biomarker for CC diagnosis.
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基因功能
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原始数据
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文献解读
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