[Expression and significance of ENPP1, TNAP and ANK proteins in the degeneration of endplate chondrocytes in rats].

OBJECTIVE:To study the expression of the pyrophosphoric acid loop's key enzymes (ENPP1, TNAP and ANK proteins) in the degeneration of rat endplate chondrocyte. METHODS:The method of enzyme digestion plus natural subculture was employed. And the primary to forth-generation cells were collected. Western blot was used to analyze the expression of ENPP1, TNAP and ANK proteins in each generation of endplate chondrocytes. RESULTS:The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually and that of the third and forth-generation decreased. CONCLUSION:While studying the correlation between the degeneration of rat endplate chondrocytes and pyrophosphoric acid loop, it is more significant to choose the cells before the third generation. Meanwhile it is possible to lower the incidence of intervertebral discs degeneration by controlling the expression of the above-mentioned proteins in endplate cartilage.

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