The E. coli single protein production system for production and structural analysis of membrane proteins.

J. Struct. Funct. Genomics. 2010 Mar;11(1):81-4. doi:10.1007/s10969-009-9070-2. Epub 2009 Oct 15

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At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.

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The E. coli single protein production system for production and structural analysis of membrane proteins.

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