[Effects of RNA interference against aggrecanase 1 gene on extracellular matrix metabolism of cultured chondrocytes in vitro].

OBJECTIVE:To study the effect of RNA interference aggrecanase-1 gene silencing on extracellular matrix metabolism of cultured rat costochondral chondrocytes. METHODS:Rat costochondral chondrocyte monolayers were obtained by microdissection and digestion. The growth and morphological changes of the chondrocytes were observed after of aggrecanase-1 gene. The mRNA expression of aggrecanase-1 was detected by RT-PCR method, and aggrecan content was determined by Western blotting. RESULTS:The specific inhibition of aggrecanase-1 by duanyu1615 produced no adverse effect on the morphology and growth of the chondrocytes. The mRNA of aggrecanase-1 decreased and aggrecan content increased significantly after transfection of the chondrocytes. CONCLUSION:Inhibition of aggrecanase-1 decreases aggrecan degradation in cultured rat chondrocytes. duanyu1615 technique can be a useful means for studying extracellular matrix metabolism in the cartilage.

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