Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs.

Bioorg. Med. Chem. Lett.2009 Jul 15;19(14):3767-70. Epub 2009 May 03

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Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding alpha-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.

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Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs.

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