例如:"lncRNA", "apoptosis", "WRKY"

Transcriptome sequencing to detect gene fusions in cancer.

Nature. 2009 Mar 5;458(7234):97-101. Epub 2009 Jan 11
Christopher A Maher 1 , Chandan Kumar-Sinha , Xuhong Cao , Shanker Kalyana-Sundaram , Bo Han , Xiaojun Jing , Lee Sam , Terrence Barrette , Nallasivam Palanisamy , Arul M Chinnaiyan
Christopher A Maher 1 , Chandan Kumar-Sinha , Xuhong Cao , Shanker Kalyana-Sundaram , Bo Han , Xiaojun Jing , Lee Sam , Terrence Barrette , Nallasivam Palanisamy , Arul M Chinnaiyan
+ et al

[No authors listed]

Author information
  • 1 Michigan Center for Translational Pathology, Ann Arbor, USA.
全文

摘要


Recurrent gene fusions, typically associated with haematological malignancies and rare bone and soft-tissue tumours, have recently been described in common solid tumours. Here we use an integrative analysis of high-throughput long- and short-read transcriptome sequencing of cancer cells to discover novel gene fusions. As a proof of concept, we successfully used integrative transcriptome sequencing to 're-discover' the BCR-ABL1 (ref. 10) gene fusion in a chronic myelogenous leukaemia cell line and the TMPRSS2-ERG gene fusion in a prostate cancer cell line and tissues. Additionally, we nominated, and experimentally validated, novel gene fusions resulting in chimaeric transcripts in cancer cell lines and tumours. Taken together, this study establishes a robust pipeline for the discovery of novel gene chimaeras using high-throughput sequencing, opening up an important class of cancer-related mutations for comprehensive characterization.