Evolutionary conserved role for TARPs in the gating of glutamate receptors and tuning of synaptic function.

Neurotransmission in the brain is critically dependent on excitatory synaptic signaling mediated by AMPA-class ionotropic glutamate receptors (AMPARs). AMPARs are known to be associated with Transmembrane AMPA receptor Regulatory Proteins In vertebrates, at least four appear to have redundant roles as obligate chaperones for AMPARs, thus greatly complicating analysis of participation in synaptic function. We have overcome this limitation by identifying and mutating the essential set of Tduanyu37s in C. elegans (STG-1 and STG-2). In Tduanyu37 mutants, AMPAR-mediated currents and worm behaviors are selectively disrupted despite apparently normal surface expression and clustering of the receptors. Reconstitution experiments indicate that both STG-1 and STG-2 can functionally substitute for vertebrate Tduanyu37s to modify receptor function. Thus, we show that Tduanyu37s are obligate auxiliary subunits for AMPARs with a primary, evolutionarily conserved functional role in the modification of current kinetics.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}