[In vitro study of the killing activities on H-2(d) murine normal and tumor cells by Ly49A gene transfected lymphocytes].

OBJECTIVE:To observe the killing activities of Ly49A gene transfected lymphocytes of C57BL/6 mice to normal and tumor cells of BALB/c mice. METHODS:pLXSN-Ly49A retrovirus vector was constructed and packaged with PA317 cell line. The lymphocytes of C57BL/6 mice were transfected by culture with virus producing PA317 cells. The Ly49A expression rate on the transfected lymphocytes was detected by flow cytometry and the killing activities of the transfected lymphocytes to normal and tumor cells of BALB/c mice were assayed by MTT method. RESULTS:The Ly49A expression rates of C57BL/6 mice lymphocytes transfected with pLXSN-Ly49A for 24 hours, of those transfected with pLXSN and nontransfected control were (46.67 +/- 0.35)%, (18.73 +/- 0.85)%, and (19.60 +/- 0.27)%, respectively. The killing activity of the transfected lymphocytes to 4T(1) tumor cells remained almost the same as that of the control (P > 0.05), but to normal fibroblasts decreased sharply (inhibiting rate 22% - 25%). CONCLUSION:The Ly49A transfected C57BL/6 mice lymphocytes could kill BALB/c mice tumor cells as effectively as the control did, but the activity decreased sharply to normal BALB/c mice cells, which would be instructive for resolving graft versus host disease after allogeneic bone marrow transplantation.

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