[No authors listed]
The S-like ribonucleases (RNases) and of Arabidopsis are members of the widespread T2 ribonuclease family, whose members also include the S-RNases, involved in gametophytic self-incompatibility in plants. Both duanyu16681 and duanyu16682 mRNAs have been shown previously to be induced by inorganic phosphate (Pi) starvation. In our study we examined this regulation at the protein level and determined the effects of diminishing duanyu16681 and duanyu16682 expression using antisense techniques. The Pi-starvation control of duanyu16681 and duanyu16682 was confirmed using antibodies specific for each protein. These specific antibodies also demonstrated that duanyu16681 is secreted, whereas duanyu16682 is intracellular. By introducing antisense constructs, mRNA accumulation was inhibited by up to 90% for duanyu16681 and up to 65% for These plants contained abnormally high levels of anthocyanins, the production of which is often associated with several forms of stress, including Pi starvation. This effect demonstrates that diminishing the amounts of either duanyu16681 or duanyu16682 leads to effects that cannot be compensated for by the actions of other RNases, even though Arabidopsis contains a large number of different RNase activities. These results, together with the differential localization of the proteins, imply that duanyu16681 and duanyu16682 have distinct functions in the plant.
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