Molecular cloning and sequence analysis of the Schizosaccharomyces pombe ade10+ gene.

Yeast. 1998 Oct;14(14):1307-10

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We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5-phosphoribosyl-4-carboxamide 5-aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupted open reading frame of 1755 nucleotides corresponding to 585 amino acid residues. The deduced amino acid sequence shows a high degree of similarity to the purH gene product of many species, including Saccharomyces cerevisiae, human, chicken and Escherichia coli. Moreover our data indicate that intrachromosomal recombination in Schiz. pombe is enhanced if the ade10 gene product is defective.

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Molecular cloning and sequence analysis of the Schizosaccharomyces pombe ade10+ gene.

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