Cloning and sequence analysis of the cDNA encoding the elongation factor-1 beta of Dictyostelium discoideum.
Biochim. Biophys. Acta. 1998 Mar 03;1383(1):1-3
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摘要
A cDNA encoding elongation factor-1 beta (DdEF-1 beta) for protein synthesis was isolated from Dictyostlium discoideum cells by the differential display (DD) method. The DdEF-1 beta cDNA sequence with a length of 733 bp encodes a protein (M(r) 24.1 kDa) consisting of 214 amino acids. Comparison of the predicted amino acid sequence revealed relatively high homology to that of DdEF-1 beta from silkworm (59%), human (57%) and Xenopus (55%). Northern analysis showed that the DdEF-1 beta mRNA is maximally expressed during the vegetative growth phase, followed by marked decrease in response to cell differentiation induced by starvation.
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基因功能
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原始数据
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文献解读
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