[No authors listed]
A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.
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higA, higB, kan, orf10, orf100, orf101, orf102, orf103, orf104, orf105, orf106, orf107, orf108, orf109, orf11, orf110, orf111, orf112, orf113, orf114, orf115, orf116, orf117, orf118, orf119, orf12, orf120, orf121, orf122, orf123, orf124, orf125, orf126, orf127, orf128, orf129, orf13, orf130, orf131, orf132, orf133, orf134, orf135, orf136, orf137, orf138, orf139, orf14, orf140, orf141, orf142, orf143, orf144, orf145, orf146, orf147, orf148, orf149, orf15, orf150, orf151, orf152, orf153, orf154, orf155, orf156, orf157, orf158, orf159, orf16, orf160, orf161, orf162, orf163, orf164, orf165, orf167, orf168, orf169, orf17, orf170, orf172, orf173, orf174, orf175, orf176, orf177, orf178, orf179, orf18, orf180, orf181, orf182, orf183, orf184, orf185, orf186, orf187, orf188, orf189, orf19, orf190, orf191, orf192, orf193, orf194, orf195, orf196, orf197, orf198, orf199, orf2, orf20, orf200, orf201, orf202, orf203, orf204, orf205, orf206, orf207, orf208, orf209, orf21, orf210, orf211, orf212, orf213, orf214, orf215, orf216, orf217, orf218, orf219, orf22, orf220, orf221, orf222, orf223, orf224, orf225, orf226, orf227, orf228, orf229, orf23, orf230, orf231, orf232, orf233, orf234, orf235, orf236, orf237, orf238, orf239, orf24, orf240, orf241, orf242, orf243, orf244, orf245, orf246, orf247, orf248, orf249, orf25, orf250, orf251, orf252, orf253, orf254, orf255, orf256, orf257, orf258, orf259, orf26, orf260, orf263, orf264, orf265, orf266, orf267, orf268, orf269, orf27, orf270, orf271, orf272, orf273, orf274, orf275, orf276, orf277, orf279, orf28, orf280, orf281, orf282, orf283, orf284, orf285, orf286, orf287, orf288, orf289, orf29, orf290, orf291, orf292, orf295, orf296, orf297, orf298, orf299, orf3, orf30, orf300, orf301, orf31, orf32, orf33, orf34, orf35, orf36, orf37, orf38, orf39, orf4, orf40, orf41, orf42, orf43, orf44, orf45, orf46, orf47, orf48, orf49, orf5, orf50, orf51, orf52, orf53, orf54, orf55, orf56, orf57, orf58, orf59, orf6, orf60, orf61, orf62, orf64, orf65, orf66, orf67, orf68, orf69, orf7, orf70, orf71, orf72, orf73, orf74, orf75, orf76, orf77, orf78, orf79, orf8, orf80, orf81, orf82, orf83, orf84, orf85, orf86, orf87, orf88, orf89, orf9, orf90, orf91, orf92, orf93, orf94, orf95, orf96, orf97, orf98, orf99, parA, parB, pvuRts1I, repA, tdi
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