[No authors listed]
Screening of a bovine seminal vesicle cDNA library in lambda gt11 with monospecific anti-ubiquitin IgGs yielded two independent clones pUF4AA and pUD4AA. Sequence analysis of pUF4AA revealed a coding region for a polyubiquitin with four tandem-repeats. We observed a replacement of serine (133) by phenylalanine in the second ubiquitin repeat. The deduced amino acid sequence of pUD4AA, however, possessed the typical serine (133) residue. This serine-phenylalanine replacement could result from allelic polymorphism.
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