Isolation and identification of cecropin antibacterial peptides from the extracellular matrix of the insect integument.

Anal. Biochem.1994 Mar;217(2):231-5

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摘要

A method for the screening and isolation of antibacterial peptides from the extracellular insect cuticle using ultrafiltration and reverse-phase high-performance liquid chromatography is presented. Using this method we were able to conveniently isolate cuticular antibacterial peptides belonging to the cecropin family in 1 day. The procedure should facilitate the rapid screening of antibacterial peptides or other bioactive peptides from extracellular matrices.

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Isolation and identification of cecropin antibacterial peptides from the extracellular matrix of the insect integument.

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