Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

J. Bacteriol.1994 Jul;176(14):4416-23. doi:10.1128/jb.176.14.4416-4423.1994

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摘要

The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.

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Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

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