A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification.

Anim. Genet.1993 Apr;24(2):125-7. doi:10.1111/j.1365-2052.1993.tb00253.x

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A polymorphism was detected in the 3' untranslated region of the bovine gamma-S-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.

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A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification.

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