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Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle myosin.

Hoppe-Seyler's Z. Physiol. Chem.1982 Nov;363(11):1321-30. doi:10.1515/bchm2.1982.363.2.1321
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摘要


The L-1 light chain of myosin, extracted from chicken fast skeletal-muscle, was digested with trypsin after S-carboxymethylation. The tryptic peptides in the digest were separated and purified and the amino-acid compositions and sequences of the tryptic peptides analysed. The carboxymethylated L-1 light chain was also digested with pepsin and the peptic peptides in the digest were separated and purified. The amino-acid compositions and the partial sequences of peptic peptides were analysed. From these results, the primary structure of the L-1 light chain of chicken fast skeletal-muscle myosin was determined. The established sequence consists of 190 amino acids.

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