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Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment.

Proc. Natl. Acad. Sci. U.S.A.1985 Jan;82(1):63-7. doi:10.1073/pnas.82.1.63
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摘要

We have isolated and characterized overlapping clones from phage lambda and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

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