Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma.

BACKGROUND:Pituitary tumor-transforming gene 1 was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear. METHODS:The expressions of and miRNA in oral SCC cell lines and their invasion capability depended on duanyu1547G1 expression were analyzed by quantitative RT-PCR, Western blots, the transwell insert system and Zymography. RESULTS:Invasion abilities were decreased in oral SCC cells treated with When duanyu1547G1 were downregulated in oral SCC cells treated with microRNA-186 and -655 inhibited their invasion abilities via MMP-9 activity. CONCLUSIONS:These results indicate that alteration of expression of duanyu1547G1 in oral SCC cells by newly identified microRNA-186 and -655 can regulate invasion activity. Therefore, these data offer new insights into further understanding duanyu1547G1 function in oral SCC and should provide new strategies for diagnostic markers for oral SCC.

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