Poly(ADP-ribose) polymerase 1 in genome-wide expression control in Drosophila.

Poly(ADP-ribose) polymerase 1 is a nuclear enzyme involved in DNA repair and transcription regulation, among other processes. Malignant transformations, tumor progression, the onset of some neuropathies and other disorders have been linked to misregulation of activity. Despite intensive studies during the last few decades, the role of Pduanyu37-1 in transcription regulation is still not well understood. In this study, a transcriptomic analysis in Drosophila melanogaster third instar larvae was carried out. A total of 602 genes were identified, showing large-scale changes in their expression levels in the absence of Pduanyu37-1 in vivo. Among these genes, several functional gene groups were present, including transcription factors and cytochrome family members. The transcription levels of genes from the same functional group were affected by the absence of Pduanyu37-1 in a similar manner. In the absence of all misregulated genes coding for transcription factors were downregulated, whereas all genes coding for members of the cytochrome P450 family were upregulated. The cytochrome P450 proteins contain heme as a cofactor and are involved in oxidoreduction. Significant changes were also observed in the expression of several mobile elements in the absence of Pduanyu37-1, suggesting that Pduanyu37-1 may be involved in regulating the expression of mobile elements.

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