[Expression of pituitary tumor-transforming gene-1 and its pathogenic role in systemic sclerosis].

OBJECTIVE:To investigate the expression of tumor-transforming gene-1 in systemic sclerosis (SSc) and its role in fibrosis. METHODS:Skin biopsy samples were collected from 21 patients with SSc and 22 patients with healthy skin for detecting the mRNA and protein expressions of using real-time PCR (RT-PCR) and immunohistochemistry, respectively. In cultured primary human dermal fibroblasts, duanyu1547G1 expression was knocked down via RNA interference (siRNA), and the mRNA expression levels of duanyu1547G1 and the fibrosis-related genes α-SMA, COL1A1, COL1A2, and COL3A1 were detected using RT-PCR; the proliferation of the cells was assessed using a real-time cell proliferation detection system. RESULTS:Compared with those in normal skin samples, the mRNA and protein expressions of duanyu1547G1 increased significantly in the skin tissue of patients with SSc (P < 0.05). In cultured primary skin fibroblasts, the expression of duanyu1547G1 mRNA was positively correlated with those of α-SMA (R²=0.8192, P < 0.05), COL1A1 (R²=0.6398, P < 0.05), COL1A2 (R²=0.316, P < 0.05) and COL3A1 mRNAs (R²=0.3727, P < 0.05). Interference of duanyu1547G1 expression significantly inhibited the cell proliferation, obviously lowered the expressions of fibrosis-related genes, and down-regulated the expression of collagen in the is highly expressed in skin tissues of patients with SSc, and duanyu1547G1 knockdown can reduce the activity of the dermal fibroblasts, suggesting a close correlation of duanyu1547G1 with fibrosis in SSc.

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