[Cathepsin S (CTSS) is highly expressed in temozolomide-resistant glioblastoma T98G cells and associated with poor prognosis].

Objective To investigate the expression of cathepsin S (CTSS) in temozolomide-resistant glioblastoma T98G (T98G-R) cells. Methods The differentially expressed genes involved in T98G-R cells were obtained from NCBI database, and the expression of CTSS in glioblastoma was analyzed in Gene Expression Profiling Interactive Analysis (GEPIA2) and Chinese Glioma Genome Atlas (CGGA) database. Real-time quantitative PCR and Western blot analysis were used to detect CTSS expression in T98G cells and T98G-R cells. The correlation between CTSS expression level and patient prognosis was analyzed using the Cancer Genome Atlas (TCGA) database. Results Gene CTSS was screened out by comparing the differentially expressed genes in T98G-R cells using NCBI GEO chip data GSE2221. GEPIA2 database analysis showed higher CTSS expression in glioblastoma tissues than in normal tissues. Both mRNA and protein levels of CTSS in T98G-R cells were significantly higher than those in T98G cells. TCGA database showed that GBM patients with high CTSS expression exhibited poorer prognosis. Conclusion CTSS is highly expressed in T98G-R cells and is associated with poor prognosis in patients.

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