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Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies.

Int J Mol Sci. 2020 Oct 30;21(21)
Annalucia Carbone 1 , Natalia Linkova 2 , Victoria Polyakova 3 , Ekaterina Mironova 4 , Ulduz Hashimova 5 , Ahmed Gadzhiev 5 , Khatira Safikhanova 5 , Tatiana Kvetnaia Julia Krylova 6 , Roberto Tarquini 7 , Gianluigi Mazzoccoli 1 , Igor Kvetnoy 8
Annalucia Carbone 1 , Natalia Linkova 2 , Victoria Polyakova 3 , Ekaterina Mironova 4 , Ulduz Hashimova 5 , Ahmed Gadzhiev 5 , Khatira Safikhanova 5 , Tatiana Kvetnaia Julia Krylova 6 , Roberto Tarquini 7 , Gianluigi Mazzoccoli 1 , Igor Kvetnoy 8
+ et al

[No authors listed]

Author information
  • 1 Department of Medical Sciences, Division of Internal Medicine and Chronobiology Laboratory, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
  • 2 Department of Therapy, Geriatrics, and Anti-Aging Medicine, Academy of Postgraduate Education under FSBU FSCC of FMBA of Russia, 125310 Moscow, Russia.
  • 3 Department of Physiology and Department of Pathology, Saint Petersburg State University, 199034 Saint Petersburg, Russia.
  • 4 Department of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, 197110 Saint Petersburg, Russia.
  • 5 Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, Baku AZ1100, Azerbaijan.
  • 6 Department of Pathology, Pavlov First Saint-Petersburg State Medical University, 197022 Saint Petersburg, Russia.
  • 7 Inter-institutional Department for Continuity of Care of Empoli, University of Florence, 50134 Florence, Italy.
  • 8 Center of Molecular Biomedicine, Saint-Petersburg Research Institute of Phthisiopulmonology, 191036 Saint Petersburg, Russia.

摘要

Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH.

KEYWORDS: aging, arterial hypertension, buccal epithelium, melatonin, sirtuins

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