The NAD⁺-mediated self-inhibition mechanism of pro-neurodegenerative SARM1.

Pathological degeneration of axons disrupts neural circuits and represents one of the hallmarks of neurodegeneration^1-4. Sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) is a central regulator of this neurodegenerative process^5-8, and its Toll/interleukin-1 receptor (TIR) domain exerts its pro-neurodegenerative action through NADase activity^9,10. However, the mechanisms by which the activation of SARM1 is stringently controlled are unclear. Here we report the cryo-electron microscopy structures of full-length SARM1 proteins. We show that NAD⁺ is an unexpected ligand of the armadillo/heat repeat motifs (ARM) domain of SARM1. This binding of NAD⁺ to the ARM domain facilitated the inhibition of the TIR-domain NADase through the domain interface. Disruption of the NAD⁺-binding site or the ARM-TIR interaction caused constitutive activation of SARM1 and thereby led to axonal degeneration. These findings suggest that NAD⁺ mediates self-inhibition of this central pro-neurodegenerative protein.

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