Effects of miR-432 and miR-548c-3p on the proliferation and invasion of osteosarcoma cells.

PURPOSE:To investigate the effects of miR-432 and miR-548c-3p on the proliferation and invasion of osteosarcoma cells. METHODS:A total of 67 cases of patients with osteosarcoma who came to the third Affiliated Hospital of Southern Medical University from April 2015 to May 2018 formed the experimental group, and 63 healthy individuals who came to this hospital for physical examination during the same period formed the control group. The expressions of miR-432 and miR-548c-3p in sera of each group were detected by RT-PCR to observe the changes of the expression levels of these miRs in the sera of the experimental and the control group, and the relationship between the expression levels of these miRs in the sera of patients with osteosarcoma and the grade of tumor differentiation and different pathological classification. GM-63 cells were selected as the target for in vitro experiments which were cultured and transfected. Before transfection, cells were divided into blank group (without transfection), negative control group (transfected with miR NC) and experimental group (transfected with miR-432 mimics/miR-548c-3p mimics). Cell proliferation was detected by CCK-8 method, cell apoptosis was detected by flow cytometry, and cell invasion by transwell invasion experiment. RESULTS:miR-423 and miR-548c-3p showed low expression in osteosarcoma, and the overexpression of miR-432 and miR-548c-3p in osteosarcoma cells could inhibit the proliferation of tumor cells and promote their apoptosis. miR-432 and miR-548c-3p might be tumor suppressors of osteosarcoma, and their expression levels could be used as important reference indexes to evaluate the benign and malignant levels of osteosarcoma. CONCLUSION:These results suggested that the abnormal expressions of miR-432 and miR-548c-3p may be key factors of the occurrence and development of osteosarcoma.

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