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The role of endothelin B receptor in bone modelling during orthodontic tooth movement: a study on ETB knockout rats.

Sci Rep. 2020 Aug 26;10(1):14226
S Ibrahimi Disha 1 , B Furlani 2 , G Drevensek 2 , A Plut 3 , M Yanagisawa 4 , S Hudoklin 5 , I Prodan Žitnik 6 , J Marc 6 , M Drevensek 7
S Ibrahimi Disha 1 , B Furlani 2 , G Drevensek 2 , A Plut 3 , M Yanagisawa 4 , S Hudoklin 5 , I Prodan Žitnik 6 , J Marc 6 , M Drevensek 7
+ et al

[No authors listed]

Author information
  • 1 Department of Orthodontics, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, 1000, Ljubljana, Slovenia.
  • 2 Institute of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
  • 3 Department of Orthodontics, University Medical Center Ljubljana, Ljubljana, Slovenia.
  • 4 International Institute for Integrative Sleep Medicine, University of Tsukuba, Tsukuba, Japan.
  • 5 Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
  • 6 Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia.
  • 7 Department of Orthodontics, University Medical Center Ljubljana, Ljubljana, Slovenia. martina.drevensek@kclj.si.

摘要


The endothelin system has an important role in bone modelling during orthodontic tooth movement (OTM); however, little is known about the involvement of endothelin B receptors (ETB) in this process. The aim of this study was to evaluate the role of ETB in bone modelling during OTM using ETB knockout rats (ETB-KO). Thirty-two male rats were divided into 4 groups (n = 8 per group): the ETB-KO appliance group, ETB-KO control group, wild type (ETB-WT) appliance group, and ETB-WT control group. The appliance consisted of a super-elastic closed-coil spring placed between the first and second left maxillary molar and the incisors. Tooth movement was measured on days 0 and 35, and maxillary alveolar bone volume, osteoblast, and osteoclast volume were determined histomorphometrically on day 35 of OTM. Next, we determined the serum endothelin 1 (ET-1) level and gene expression levels of the osteoclast activity marker cathepsin K and osteoblast activity markers osteocalcin and dentin matrix acidic phosphoprotein 1 (DMP1) on day 35. The ETB-KO appliance group showed significantly lower osteoblast activity, diminished alveolar bone volume and less OTM than the ETB-WT appliance group. Our results showed that ETB is involved in bone modelling in the late stage of OTM.