Nuclear transporter karyopherin subunit alpha 3 levels modulate Porcine circovirus type 2 replication in PK-15 cells.

Entering the nucleus is important for Porcine circovirus type 2 (PCV2) replication. Karyopherins (KPNs) mediate the nuclear import of many cytoplasmic proteins. Our previous study showed that is involved in interferon production during PCV2 infection induced by Poly I:C and ISD (Interferon stimulatory DNA). However, it remains unclear whether PCV2 replication is associated with In the present study, knockdown of Kduanyu15353 promoted the replication of PCV2, whereas overexpression of Kduanyu15353 inhibited PCV2 replication in PK-15 cells. Furthermore, Kduanyu15353 knockdown inhibited IRF3 and reduced the expression of antiviral genes including IFN-β, ISG54, Mx1 and ISG56, while the opposite results were obtained after Kduanyu15353 overexpression. Kduanyu15353 knockdown also promoted p65 nuclear translocation and increased the mRNA expression of IL-10 and IL-1β. These results suggested that Kduanyu15353 facilitates IRF3 entry into the nucleus and the production of an antiviral response, resulting in PCV2 replication inhibition and blockage of NF-κB signal activation.

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