Cloning and expression of the Escherichia coli K-12 sad gene.

J. Bacteriol.1988 Feb;170(2):991-4. doi:10.1128/jb.170.2.991-994.1988

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摘要

The Escherichia coli K-12 sad gene, which encodes an NAD-dependent succinic semialdehyde dehydrogenase, was cloned into a high-copy-number vector. Minicells carrying a sad+ plasmid produced a 55,000-dalton peptide, the probable sad gene product.

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Cloning and expression of the Escherichia coli K-12 sad gene.

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