例如:"lncRNA", "apoptosis", "WRKY"

Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry.

PLoS Biol. 2020 Aug 06;18(8):e3000762. eCollection 2020 Aug
Emmanuel Gallaud 1 , Anjana Ramdas Nair 1 , Nicole Horsley 2 , Arnaud Monnard 2 , Priyanka Singh 1 , Tri Thanh Pham 2 , David Salvador Garcia 1 , Alexia Ferrand 1 , Clemens Cabernard 2
Emmanuel Gallaud 1 , Anjana Ramdas Nair 1 , Nicole Horsley 2 , Arnaud Monnard 2 , Priyanka Singh 1 , Tri Thanh Pham 2 , David Salvador Garcia 1 , Alexia Ferrand 1 , Clemens Cabernard 2
+ et al

[No authors listed]

Author information
  • 1 Biozentrum, University of Basel, Klingelbergstrasse, Basel, Switzerland.
  • 2 Department of Biology, University of Washington, Life Science Building, Seattle, Washington State, United States of America.

摘要


Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older "mother" and a younger "daughter" centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb's asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.