[No authors listed]
Gestational diabetes mellitus (GDM) is a type of diabetes mellitus (DM) that occurs during pregnancy. The present study aimed to investigate the roles of microRNA (miR)â195â5p and enhancer of zeste homolog 2 (EZH2) in GDM, and their potential association. Human umbilical vein endothelial cells (HUVECs) were collected from healthy and GDM umbilical cords, and the endothelial properties were detected by flow cytometry. mRNA expression levels of miRâ195â5p and EZH2, and EZH2 protein expression levels were detected by reverse transcriptionâquantitative PCR (RTâqPCR) and western blot analysis, respectively. Cell colony formation and flow cytometry were performed to determine cell proliferation and apoptosis. Furthermore, the target gene of miRâ195â5p was predicted and assessed using a dualâluciferase reporter assay. The levels of cell viability, proliferation and apoptosis following the overexpression of miRâ195â5p, EZH2 or miRâ195â5p + EZH2, were detected using Cell Counting Kitâ8, colony formation and flow cytometry assays, respectively. In addition, the mRNA expression levels of miRâ195â59 and EZH2, and EZH2 protein expression levels following transfection with overexpression plasmids were detected using RTâqPCR and western blot analysis, respectively. It was identified that high mRNA expression of miRâ195â5p, and low EZH2 mRNA and protein expression levels decreased the level of cell proliferation and the high apoptotic rate of GDMâHUVECs. In addition, miRâ195â5p was predicted and identified to target EZH2, and miRâ195â5p overexpression was identified to inhibit cell proliferation and promote apoptosis. However, it was demonstrated that upregulation of EZH2 could alleviate the inhibition of cell proliferation and the increased apoptotic rate induced by miRâ195â5p overexpression. Therefore, the present results suggested that miRâ195â5p may inhibit cell viability, proliferation and promote apoptosis by targeting EZH2 in GDMâinduced HUVECs.
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