ACYP2 contributes to malignant progression of glioma through promoting Ca²⁺ efflux and subsequently activating c-Myc and STAT3 signals.

BACKGROUND:Acylphosphatase 2 (ACYP2) is involved in cell differentiation, energy metabolism and hydrolysis of intracellular ion pump. It has been reported as a negative regulator in leukemia and a positive regulator in colon cancer, respectively. However, its biological role in glioma remains totally unclear. METHODS:We performed quantitative RT-PCR (qRT-PCR), immunohistochemistry (IHC) and western blot assays to evaluate ACYP2 expression. The functions of ACYP2 in glioma cells were determined by a series of in vitro and in vivo experiments, including cell proliferation, colony formation, cell cycle, apoptosis, migration, invasion and nude mouse tumorigenicity assays. In addition, western blot and co-immunoprecipitation (Co-IP) assays were used to identify its downstream targets. RESULTS:Knocking down ACYP2 in glioma cells significantly inhibited cell proliferation, colony formation, migration, invasion and tumorigenic potential in nude mice, and induced cell cycle arrest and apoptosis. Conversely, ectopic expression of ACYP2 in glioma cells dramatically promoted malignant phenotypes of glioma cells. Mechanistically, ACYP2 promoted malignant progression of glioma cells through regulating intracellular Ca²⁺ homeostasis via its interaction with PMCA4, thereby activating c-Myc and signals. This could be effectively reversed by Ca²⁺ chelator BAPTA-AM or calpain inhibitor calpeptin. CONCLUSIONS:Our data demonstrate that ACYP2 functions as an oncogene in glioma through activating c-Myc and signals via the regulation of intracellular Ca²⁺ homeostasis, and indicate that ACYP2 may be a potential therapeutic target and prognostic biomarker in gliomas.

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