miR-874 inhibits gastric cancer cell proliferation by targeting SPAG9.

BACKGROUND:microRNAs (miRNAs) play essential roles in the development and progression of gastric cancer (GC). Although aberrant miR-874 expression has been reported in various human cancers, its role in GC remains obscure. METHODS:miR-874 expression was assessed by real-time quantitative polymerase chain reaction (RT-qPCR) in 62 matched GC and adjacent normal tissues, as well as in GC cell lines and immortalized human gastric epithelial cells. CCK8 assay, colony formation assay, and flow cytometry were used to assess the role of miR-874 in GC cell proliferation and apoptosis in vitro. Additionally, to determine the effects of miR-874 on GC cell proliferation and apoptosis in vivo, BALB/c nude mice were injected with GC cells transfected with a miR-874 mimic. The role of miR-874 in expression was assessed by luciferase assay, Western blotting, and RT-qPCR. RESULTS:miR-874 was downregulated in GC cell lines and tissues. miR-874 overexpression in GC cells led to inhibition of cell proliferation and induction of apoptosis. Moreover, duanyu1842G9 was identified as a direct miR-874 target, the expression of which was suppressed by miR-874. duanyu1842G9 overexpression markedly promoted GC cell proliferation. CONCLUSIONS:miR-874 inhibited cell proliferation and induced apoptosis in GC cells. duanyu1842G9 downregulation was crucial for the tumor-suppressive effects of miR-874. Hence, the axis could serve as a novel therapeutic target in GC.

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