LncRNA MCM3AP-AS1 promotes breast cancer progression via modulating miR-28-5p/CENPF axis.

Breast cancer is one of the commonly occurred cancers among women and poses a huge threat against female health. Abnormal expression of lncRNA has been confirmed to be an important inducer of cancer. By searching GEO and TCGA database, we found that CENPF was upregulated in breast cancer tissues. Through RT-qPCR, CENPF was found to be upregulated in breast cancer cells. Functional experiments revealed that CENPF had positive effect on the cellular functions, including proliferation, migration and invasion. Subsequently, CENPF was confirmed to combine with miR-28-5p, and its expression was suppressed by miR-28-5p. Furthermore, it was found that miR-28-5p bound to MCM3AP-AS1, and MCM3AP-AS1 expressed at a high level in breast cancer cells. Besides, MCM3AP-AS1 was confirmed as a cytoplasmic RNA. In addition, there was a positive expression correlation between MCM3AP-AS1 and CENPF. Therefore, MCM3AP-AS1 was confirmed to regulate CENPF via competitively binding to miR-28-5p. At last, rescue assays demonstrated that knockdown of CENPF restored miR-28-5p repression-induced cellular processes in MCM3AP-AS1-silenced cells. In vivo assay revealed that MCM3AP-AS1 could hasten tumor growth in breast cancer by targeting CENPF. All results indicated that MCM3AP-AS1/miR-28-5p/CENPF axis accelerates breast cancer progression.

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